Effect of Inhibitors of Distinct Signaling Pathways on Neutrophil O2− Generation in Response to Tumor Necrosis Factor-α, and Antibodies Against CD18 and CD11a: Evidence for a Common and Unique Pattern of Sensitivity to Wortmannin and Protein Tyrosine
Effect of Inhibitors of Distinct Signaling Pathways on Neutrophil O2− Generation in Response to Tumor Necrosis Factor-α, and Antibodies Against CD18 and CD11a: Evidence for a Common and Unique Pattern of Sensitivity to Wortmannin and Protein Tyrosine
复制标题
不同信号通路抑制剂对肿瘤坏死因子-α 以及抗 CD18 和 CD11a 抗体的中性粒细胞 O2− 生成的影响：对渥曼青霉素和蛋白酪氨酸的常见和独特敏感性模式的证据
DOI：
--
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
G. Berton
中科院分区：
文献类型：
--
作者：
C. Laudanna;F. Rossi;G. Berton
摘要 在这些研究中，我们表明，肿瘤坏死因子-α (TNF) 或针对白细胞整合素 (CD 18) 共同 β2 链的抗体或 LFA-1 (CD 11 a) 刺激 O2− 产生，显示出对不同信号通路抑制剂的敏感性或不敏感性的常见且独特的模式。刺激中性粒细胞产生 O2− 的两种方式均被渥曼青霉素、肌球蛋白轻链激酶抑制剂（Nakanishi, S.,et al.,1992.,J. Biol. Chem. 267, 2157-2163）和三种不同的蛋白酪氨酸激酶抑制剂阻断。无论是星形孢菌素（一种蛋白激酶 C 抑制剂）还是百日咳毒素，其浓度均不会抑制 PMA 和 FMLP 产生的 O2− 生成。分别有什么影响。
Abstract In these studies we show that stimulation of O2− generation by Tumor necrosis factor-α (TNF), or antibodies against the common β2 chain of leukocyte integrins (CD 18), or LFA-1 (CD 11 a) displays a common and unique pattern of sensitivity or insensitivity to inhibitors of different signalling pathways. Both ways of stimulating neutrophil O2− generation were blocked by wortmannin, an inhibitor of myosin light chain kinase (Nakanishi, S.,et al. ,1992.,J. Biol. Chem. 267, 2157-2163), and three different inhibitors of protein tyrosine kinases. Neither staurosporine, a protein kinase C inhibitor, nor Pertussis toxin, at concentrations which inhibited O2− generation in response to PMA, and FMLP. respectively, had any effect.