Identification of metabolites of 2, 3, 7, 8-tetrachlorodibenzo-P-dioxin (TCDD) formed on incubation with isolated rat hepatocytes.
Identification of metabolites of 2, 3, 7, 8-tetrachlorodibenzo-P-dioxin (TCDD) formed on incubation with isolated rat hepatocytes.
复制标题
鉴定与分离的大鼠肝细胞孵育时形成的 2,3,7,8-四氯二苯并-P-二恶英 (TCDD) 的代谢物。
DOI：
10.1016/s0006-291x(82)80050-8
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发表时间：
1982
影响因子：
3.1
通讯作者：
Neal,RA
中科院分区：
文献类型：
--
作者：
Sawahata,T;Olson,JR;Neal,RA
将氚化TCDD与分离的大鼠肝细胞孵育，并将所得孵育混合物进行高效液相色谱（HPLC）。将不对应于TCDD的放射性主峰与β-葡萄糖醛酸酶孵育。该反应的产物用重氮甲烷衍生化，并再次进行HPLC。发现通过该程序分离的两种放射性代谢物与真实的1-甲氧基-2，3，7，8-四氯二苯并-对-二恶英和8-甲氧基-2，3，7-三氯二苯并-对-二恶英共迁移。还通过气相色谱-质谱法确认了这些代谢物的身份。
Tritiated TCDD was incubated with isolated rat hepatocytes and the resulting incubation mixture subjected to high performance liquid chromatography (HPLC). The major peak of radioactivity not corresponding to TCDD was incubated with β-glucuronidase. The products of this reaction were derivatized with diazomethane and again subjected to HPLC. Two of the radioactive metabolites isolated by this procedure were found to co-migrate with authentic 1-methoxy-2,3,7,8-tetrachlorodibenzo-p-dioxin and 8-methoxy-2,3,7-trichlorodibenzo-p-dioxin. The identity of these metabolites was also confirmed by gas chromatography-mass spectrometry.