Determination of methylated arginines in serum by a dispersive solid-phase extraction coupled with capillary electrophoresis-laser induced fluorescence detection
Determination of methylated arginines in serum by a dispersive solid-phase extraction coupled with capillary electrophoresis-laser induced fluorescence detection
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分散固相萃取-毛细管电泳-激光诱导荧光检测法测定血清中甲基化精氨酸
DOI：
10.7523/j.issn.2095-6134.2020.04.005
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发表时间：
2020-07
期刊：
影响因子：
--
通讯作者：
丁永胜
中科院分区：
文献类型：
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作者：
丁永胜
我们描述了分散固相离子交换萃取-毛细管电泳-激光诱导荧光检测法测定血清样品中的不对称二甲基精氨酸，对称二甲基精氨酸，和单甲基精氨酸。将甲基化的麻黄碱提取的树脂珠加入到4-氯-7-硝基苯并呋喃乙腈溶液和碱性硼酸钠溶液的混合溶液（pH=10）中。5)在60℃水浴中进行衍生化。在熔融石英毛细管柱中，以80 mmol/L磷酸盐（pH=2）为分离电解质，对3种衍生物进行基线分离。0)分离电压为20 kV。根据FDA生物分析方法验证指南，进行了方法验证，结果令人满意。方法已用于当地医院血清标本中三种甲基化精氨酸的测定。
We describe a dispersive solid-phase ion exchange extraction method combined with capillary electrophoresis-laser induced fluorescence detection for the determination of asymmetric dimethylarginine, symmetric dimethylarginine, and monomethylarginine in serum samples. The methylated arginine-extracted resin beads were added into a mixed solution of 4-chloro-7-nitrobenzofuran acetonitrile solution and alkaline sodium borate solution (pH=10. 5) for the derivatization in a 60℃ water bath. The three derivatives were subjected to baseline separation in a fused silica capillary under the conditions of a separation electrolyte of 80 mmol/L phosphate (pH=2. 0) and a separation voltage of 20 kV. On the basis of the FDA bioanalytical method validation guidance, the method validation was conducted and the results were satisfactory. This method was used to determine the three methylated arginines in the serum samples from local hospital.