Detection of Antibodies Against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii in Horses From Costa Rica
Detection of Antibodies Against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii in Horses From Costa Rica
复制标题
DOI：
10.1645/ge-2722.1
复制
发表时间：
2011-06-01
影响因子：
1.3
通讯作者：
Howe, D. K.
中科院分区：
文献类型：
--
作者：
Dangoudoubiyam, S.;Oliveira, J. B.;Howe, D. K.
分别使用表面抗原 (SAG) SnSAG2 酶联免疫吸附测定 (ELISA)、NhSAG1 ELISA 和改良凝集试验，检查来自中美洲哥斯达黎加的 315 匹马的血清样本中是否存在抗肉孢子虫、新孢子虫和弓形虫抗体。抗-S。通过使用 SnSAG2 ELISA，在 42.2% 的马中发现了神经元抗体。抗新孢子虫属通过使用 NhSAG1 ELISA，仅在 3.5% 的马中发现了抗体，并且这些马中只有 1 匹通过蛋白质印迹证实为血清阳性。 34.0% 的测试马中发现了弓形虫抗体，高于北美和南美之前的报告。抗S的发现。来自有袋动物广泛分布的地理区域的马体内的神经元抗体表明，有袋动物是这种寄生虫的潜在最终宿主，也是这些马的感染源。
Serum samples from 315 horses from Costa Rica, Central America, were examined for the presence of antibodies against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii by using the surface antigen (SAG) SnSAG2 enzyme-linked immunosorbent assay (ELISA), the NhSAG1 ELISA, and the modified agglutination test, respectively. Anti-S. neurona antibodies were found in 42.2% of the horses by using the SnSAG2 ELISA. Anti-Neospora spp. antibodies were found in only 3.5% of the horses by using the NhSAG1 ELISA, and only 1 of these horses was confirmed seropositive by Western blot. Antibodies to T gondii were found in 34.0% of the horses tested, which is higher than in previous reports from North and South America. The finding of anti-S. neurona antibodies in horses from geographical areas where Didelphis marsupials has wide distribution suggests that D. marsupialis is a potential definitive host for this parasite and a source of infection for these horses.