The renal glomerulus and vasculature in 'aggregation' chimeric mice.
The renal glomerulus and vasculature in 'aggregation' chimeric mice.
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“聚集”嵌合小鼠的肾小球和脉管系统。
DOI：
10.1159/000049062
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发表时间：
2002
期刊：
影响因子：
2.5
通讯作者：
Goldowitz,Daniel
中科院分区：
文献类型：
--
作者：
Gattone2nd,VincentH;Goldowitz,Daniel
在肾小球发育中，肾小球上皮细胞来源于S形肾泡的下袢。然而，目前尚不清楚毛细血管内皮是否直接来源于血管发生（例如直接从局部后肾间充质分化而来）或是否来源于血管生成（即来源于后肾中预先存在的血管系统）。这个问题已经在其他实验室使用手术创建的嵌合肾模型系统得到解决。在本研究中，通过将来自小家鼠（Mus musculus）的4-至8-细胞胚胎的细胞与来自小家鼠（Mus caroli）的细胞聚集并将聚集的胚胎植入假孕宿主中来开发嵌合肾[Goldowitz D：Neuron 1989; 3：705-713]。将种属特异性DNA克隆与原位杂交结合使用以鉴定细胞的种属来源。种间聚集体嵌合体具有不同比例的来自小家鼠和小家鼠的肾细胞;然而，确定了肾小管和鲍曼氏囊或壁上皮细胞来自一个种属的区域，而血管内皮和肾小管中的细胞来自另一个种属。然而，在这些区域，肾小球总是含有来自两个物种的细胞混合物;许多肾小球内皮细胞似乎与血管内皮细胞和间质细胞来自同一物种。这些发现支持了血管生成可能有助于形成肾小球毛细血管内皮细胞的假设。大多数肾内动脉含有来自两个物种的细胞。然而，发现少数血管中的内皮细胞来自一个物种，而平滑肌细胞来自另一个物种。这一发现表明，肾内动脉的发育有两个起源细胞：内皮管发育，并被形成图尼卡中膜的间充质细胞包围。聚集嵌合小鼠肾脏可能成为一个有用的模型系统，在原位研究肾脏发育中涉及的复杂过程。
In glomerular development, the glomerular epithelium is derived from the lower loop of the S-shaped renal vesicle. However, it is unclear whether the capillary endothelium is derived directly by vasculogenesis (eg differentiated directly from local metanephric mesenchyme) or whether they are derived by angiogenesis (ie derived from pre-existing vasculature in the metanephros). This question has been addressed in other laboratories using surgically created chimeric kidney model systems. In the present study, chimeric kidneys were developed by aggregating the cells from 4-to 8-cell embryos from Mus musculus with ones from Mus caroli and implanting the aggregated embryos into pseudopregnant hosts [Goldowitz D: Neuron 1989; 3: 705–713]. Species specific DNA clones were used in conjunction with in situ hybridization to identify the species origin of cells. Interspecies aggregate chimeras had varying proportions of renal cells derived from Mus caroli and Mus musculus; however, regions were identified in which the renal tubular and Bowman’s capsule or parietal epithelia were from one species while vessel endothelium and cells in the interstitium were from the other species. In those regions, glomeruli always contained an admixture of cells from both species however; many of the glomerular endothelial cells appear to be from the same species as the vessel endothelium and interstitial cells. These findings support the hypothesis that angiogenesis may contribute cells that help form the glomerular capillary endothelium. Most intrarenal arteries contained cells from both species. However a few vessels were found in which the endothelium was derived from one species while the smooth muscle cells were from the other species. This finding suggests that intrarenal arterial development has two cells of origin: the endothelial tube develops and is surrounded by mesenchymal cells that form the tunica media. The aggregation chimeric mouse kidney may become a useful model system for studying in situ aspects of the complex processes involved in kidney development.