An anatomic gene expression atlas of the adult mouse brain
An anatomic gene expression atlas of the adult mouse brain
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DOI：
10.1038/nn.2281
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发表时间：
2009-03-01
影响因子：
25
通讯作者：
Hawrylycz, Michael
中科院分区：
文献类型：
--
作者：
Ng, Lydia;Bernard, Amy;Hawrylycz, Michael
研究基因表达为理解神经系统中的结构-功能关系提供了有力的手段。基因组规模的原位杂交数据集的可用性为基于基因表达模式理解大脑组织提供了新的可能性。解剖基因表达图谱（AGEA）是一种新的关系图谱，其基于艾伦脑图谱（阿坝）中数千个基因的表达数据之间的空间相关性，揭示了成年C57 Bl/6 J小鼠脑的遗传结构。AGEA包括三种用于检查神经解剖学关系和边界的发现工具：（1）基于表达的三维相关图，（2）基于转录组的大脑分层，以及（3）从阿坝中检索特定基因的设施，这些基因在局部相关域中显示丰富的表达。该图谱的实用性通过对丘脑、纹状体和大脑皮层的遗传组织的分析来说明。AGEA是与阿坝集成的可公开访问的在线计算工具（http：//mouse.brain-map.org/agea）。
Studying gene expression provides a powerful means of understanding structure-function relationships in the nervous system. The availability of genome-scale in situ hybridization datasets enables new possibilities for understanding brain organization based on gene expression patterns. The Anatomic Gene Expression Atlas (AGEA) is a new relational atlas revealing the genetic architecture of the adult C57Bl/6J mouse brain based on spatial correlations across expression data for thousands of genes in the Allen Brain Atlas (ABA). The AGEA includes three discovery tools for examining neuroanatomical relationships and boundaries: (1) three-dimensional expression-based correlation maps, (2) a hierarchical transcriptome-based parcellation of the brain and (3) a facility to retrieve from the ABA specific genes showing enriched expression in local correlated domains. The utility of this atlas is illustrated by analysis of genetic organization in the thalamus, striatum and cerebral cortex. The AGEA is a publicly accessible online computational tool integrated with the ABA (http://mouse.brain-map.org/agea).