Heterogeneous nuclear RNA promotes synthesis of (2',5')oligoadenylate and is cleaved by the (2',5')oligoadenylate-activated endoribonuclease.
Heterogeneous nuclear RNA promotes synthesis of (2',5')oligoadenylate and is cleaved by the (2',5')oligoadenylate-activated endoribonuclease.
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异质核 RNA 促进 (2,5) 寡腺苷酸的合成，并被 (2,5) 寡腺苷酸激活的核糖核酸内切酶裂解。
DOI：
10.1128/mcb.2.2.154-160.1982
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发表时间：
1982
影响因子：
5.3
通讯作者：
Baglioni,C
中科院分区：
文献类型：
--
作者：
Nilsen,TW;Maroney,PA;Robertson,HD;Baglioni,C
Heterogeneous nuclear RNA contains double-stranded regions that are not found in mRNA and that may serve as recognition elements for processing enzymes. The double-stranded regions of heterogeneous nuclear RNA prepared from HeLa cells promoted the synthesis of (2′,5′)oligoadenylate [(2′,5′)oligo(A) or (2′5′)An] when incubated with (2′,5′)Anpolymerase. This enzyme is present in elevated levels in interferon-treated cells, and labeled heterogeneous nuclear RNA incubated with extracts of these cells is preferentially cleaved, since mRNA included in the same incubations is not appreciably degraded. The cleavage of heterogenous nuclear RNA is caused by the synthesis of (2′5′)Anand by a “localized” activation of the (2′,5′)An-dependent endonuclease, since it was enhanced by ATP, the substrate of the (2′,5′)Anpolymerase, and inhibited by 2′-dATP and ethidium bromide. Both of these compounds suppress the synthesis of (2′,5′)An, the first by competitive inhibition and the latter by intercalating into double-stranded RNA. The possible role of double-stranded regions and of the (2′,5′)Anpolymerase-endonuclease system in the processing of heterogeneous nuclear RNA is discussed.
