Rhizobium oryzicola sp. nov., potential plant-growth-promoting endophytic bacteria isolated from rice roots.
Rhizobium oryzicola sp. nov., potential plant-growth-promoting endophytic bacteria isolated from rice roots.
复制标题
DOI：
10.1099/ijs.0.000358
复制
发表时间：
2015-09
影响因子：
2.8
通讯作者：
Xiaoxia Zhang;Ju-sheng Gao;Yanhua Cao;Rizwan Ali Sheirdil;Xiu-Cheng Wang;Lei Zhang
中科院分区：
文献类型：
--
作者：
Xiaoxia Zhang;Ju-sheng Gao;Yanhua Cao;Rizwan Ali Sheirdil;Xiu-Cheng Wang;Lei Zhang
从水稻-水稻-紫云英轮作长期试验中表面灭菌的水稻根中分离到细菌ZYY 136（T）和ZYY 9。菌株ZYY 136（T）和ZYY 9与Rhizobium tarimense PL-41（T）的16 S rRNA基因序列相似性最高，达97.0%。管家基因recA，thrC和atpD的序列分析清楚地区分了目前描述的根瘤菌属的物种的分离物。ZYY 136（T）与ZYY 9的DNA-DNA同源性为82.3%，其中ZYY 136（T）与模式菌株R的DNA-DNA同源性为34.0%。tarimense PL-41（T）。菌株ZYY 136（T）的DNA G+C含量为58.1mol%。主要的细胞脂肪酸为特征8（C18：1ω 7 c和/或C18：1ω6c）、C16：0和C16：0 3-OH。菌株ZYY 136（T）和ZYY 9可以通过几个表型特征与以前定义的根瘤菌属的种区分开。因此，我们得出结论，菌株ZYY 136（T）和ZYY 9代表根瘤菌属的一个新种，建议将其命名为Rhizobium oryzicola sp. nov.（模式菌株ZYY 136（T）= ACCC 05753（T）= KCTC 32088（T））。
Bacterial strains ZYY136(T) and ZYY9 were isolated from surface-sterilized rice roots from a long-term experiment of rice-rice--Astragalus sinicus rotation. The 16S rRNA gene sequences of strains ZYY136(T) and ZYY9 showed the highest similarity, of 97.0%, to Rhizobium tarimense PL-41(T). Sequence analysis of the housekeeping genes recA, thrC and atpD clearly differentiated the isolates from currently described species of the genus Rhizobium. The DNA-DNA relatedness value between ZYY136(T) and ZYY9 was 82.3%, and ZYY136(T) showed 34.0% DNA-DNA relatedness with the most closely related type strain, R. tarimense PL-41(T). The DNA G+C content of strain ZYY136(T) was 58.1 mol%. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and C16 : 0 3-OH. Strains ZYY136(T) and ZYY9 could be differentiated from the previously defined species of the genus Rhizobium by several phenotypic characteristics. Therefore, we conclude that strains ZYY136(T) and ZYY9 represent a novel species of the genus Rhizobium, for which the name Rhizobium oryzicola sp. nov. is proposed (type strain ZYY136(T) = ACCC 05753(T) = KCTC 32088(T)).