Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants.
Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants.
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DOI：
10.1111/j.1600-051x.2012.01856.x
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发表时间：
2012-05
影响因子：
6.7
通讯作者：
O'Brien K
中科院分区：
文献类型：
--
作者：
Kumar PS;Mason MR;Brooker MR;O'Brien K
虽然已经确定种植体周围炎是一种细菌引起的疾病，但对健康和疾病中种植体周围社区的细菌概况知之甚少。本研究的目的是检查健康和疾病中植入物周围微生物组的微生物特征。龈下和粘膜下菌斑样本收集自40名牙周炎、种植体周围炎、牙周和种植体周围健康的受试者，并使用16S焦磷酸测序进行分析。种植体周围的生物膜表现出显着较低的多样性比龈下生物膜在健康和疾病，但是，几个物种，包括以前未知的和未知的生物体，是独特的这个利基。种植体周围菌群中优势菌属为丁酸弧菌属、弯曲杆菌属、真杆菌属、普雷沃菌属、月单胞菌属、链球菌属、放线菌属、纤毛菌属、丙酸杆菌属、消化球菌属、乳球菌属和密螺旋体属。与健康种植体相比，种植体周围疾病与较低水平的普雷沃菌属和纤毛菌属以及较高水平的放线菌属、消化球菌属、弯曲杆菌属、非变形链球菌属、丁酸弧菌属和变形链球菌属相关。与牙周炎相关的生物膜相比，这些群落还表现出较低水平的普雷沃氏菌属、非变异链球菌属、乳杆菌属、月形单胞菌属、纤毛菌属、放线菌属和较高水平的消化球菌属、支原体属、真杆菌属、弯曲杆菌属、丁酸弧菌属、变异链球菌属和密螺旋体属。种植体周围的微生物组在健康和疾病方面与牙周社区显着不同。种植体周围炎是一种以革兰氏阴性菌为主的微生物异质性感染，其复杂性低于牙周炎。
Although it is established that peri-implantitis is a bacterially induced disease, little is known about the bacterial profile of peri-implant communities in health and disease. The purpose of the present investigation was to examine the microbial signatures of the peri-implant microbiome in health and disease. Subgingival and submucosal plaque samples were collected from forty subjects with periodontitis, peri-implantitis, periodontal and peri-implant health and analyzed using 16S pyrosequencing. Peri-implant biofilms demonstrated significantly lower diversity than subgingival biofilms in both health and disease, however, several species, including previously unsuspected and unknown organisms, were unique to this niche. The predominant species in peri-implant communities belonged to the genera Butyrivibrio, Campylobacter, Eubacterium, Prevotella, Selenomonas, Streptococcus, Actinomyces, Leptotrichia, Propionibacterium, Peptococcus, Lactococcus and Treponema. Peri-implant disease was associated with lower levels of Prevotella and Leptotrichia and higher levels of Actinomyces, Peptococcus, Campylobacter, non-mutans Streptococcus, Butyrivibrio, and Streptococcus mutans than healthy implants. These communities also demonstrated lower levels of Prevotella, non-mutans Streptococcus, Lactobacillus, Selenomonas, Leptotrichia, Actinomyces and higher levels of Peptococcus, Mycoplasma, Eubacterium, Campylobacter, Butyrivibrio, Streptococcus mutans, and Treponema when compared to periodontitis-associated biofilms. The peri-implant microbiome differs significantly from the periodontal community in both health and disease. Peri-implantitis is a microbially heterogeneous infection with predominantly gram-negative species, and is less complex than periodontitis.