Diffusion based analysis in a sheath flow microchannel: the sheath flow T-sensor.
Diffusion based analysis in a sheath flow microchannel: the sheath flow T-sensor.
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鞘流微通道中基于扩散的分析：鞘流 T 传感器。
DOI：
10.1039/b501035g
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Yager,Paul
中科院分区：
文献类型：
--
作者：
Munson,MatthewS;Hawkins,KennethR;Hasenbank,MelissaS;Yager,Paul
本文描述了一种微流体通道，允许对吸附物质进行基于扩散的分析，而无需钝化通道表面。鞘流配置用于通过分析两种不同核心流体之间的界面处的强度分布的导数，从微通道内的空间分布测量荧光标记物质的扩散系数。对小分子（罗丹明 B）和中等大小的蛋白质（麦芽凝集素）进行了测量，证明了鞘流 T 传感器的实用性。
This paper describes a microfluidic channel that allows for diffusion-based analysis of adsorbing species without passivation of the channel surfaces. The sheath flow configuration was used to measure the diffusion coefficient of fluorescently labeled species from their spatial distribution within the microchannel by analyzing the derivative of the intensity profile at the interface between two distinct core fluids. Measurements for both a small molecule (rhodamine B) and an intermediate-sized protein (wheat germ agglutinin) were made, demonstrating the utility of the sheath flow T-sensor.