Magnetic hydrothermal carbonaceous nanospheres bonded cell membranes as a stable and reusable platform for discovering natural bioactive components
Magnetic hydrothermal carbonaceous nanospheres bonded cell membranes as a stable and reusable platform for discovering natural bioactive components
复制标题
DOI：
10.1016/j.cej.2022.140238
复制
发表时间：
2022-11
影响因子：
15.1
通讯作者：
Shan Liu;Pingyu Ye;Zhenqi Sang;Wanghui Gong;Peng Sun;Sha Tao;Qiaoyan Zhang;Lupin Qin;Qiming Zhao
中科院分区：
文献类型：
--
作者：
Shan Liu;Pingyu Ye;Zhenqi Sang;Wanghui Gong;Peng Sun;Sha Tao;Qiaoyan Zhang;Lupin Qin;Qiming Zhao
水热碳纳米材料的共价仿生修饰可以克服现有化学功能化的缺点，实现稳定、可持续的选择性识别能力，在萃取分离工程中具有巨大的应用潜力。本文通过葡萄糖在磁性纳米球表面的水热碳化反应和成骨细胞膜末端氨基与磁性纳米球表面羧基的水溶液酰胺化反应，制备了新型的磁性纳米球与成骨细胞膜双重功能化的磁性纳米球。由于药物与膜受体的特异性结合，CMs结合磁性HCNs（CM-MHCNs）被用作药物发现平台，从传统中药中筛选潜在的抗骨质疏松活性成分。在MN表面引入水热碳质层后，MN的吸水性、抗氧化性、抗酸性和CM接枝能力都有明显提高，并具有良好的磁性能。这些与制备的MHCN和CM之间的共价键结合提供了增强的稳定性，其中与吸附MHCN的CM（17.9 mg g−1）和MN（18.7 mg g−1）相比，损失量（7.0 mg g − 1）显著降低。CM-MHCN具有吸附能力强、吸附动力学快、选择性好和重复使用性好等特点。因此，从蛇床子中成功地发现了两个新的潜在抗骨质疏松化合物：异茴芹素和花椒毒酚，并通过药效学实验进行了验证。通过分子对接和western blot分析进一步研究了它们可能的膜受体。CM-MHCNs的研究为稳定生物材料的绿色设计提供了新的思路，并在药物先导物的发现方面展现了良好的前景，从而促进了传统中药的发展。
The covalent bionic modifications of hydrothermal carbonaceous nanomaterials (HCNs) are of great potential in extraction and separation engineering because they can avoid present disadvantages of chemical functionalization and achieve selective recognition capacity with stability and sustainability. This article developed novel dual functionalized HCNs with sealed magnetic nanospheres (MNs) and cell membranes (CMs) of osteoblast, via the hydrothermal carbonization of glucose on MN surface and the aqueous amidation between terminal amino groups on CMs and surface carboxyl groups on HCNs. Owing to the specific combinations between drugs and membrane receptors, CMs bonded magnetic HCNs (CM-MHCNs) were applied as a drug discovery platform to screen potential anti-osteoporosis components from traditional herb medicine. The introduction of hydrothermal carbonaceous layer on MNs was proved to sharply improve their water dispersibility, resistance to oxidation and acids and CM grafting capacity with good magnetic property. These combined with the covalent bonding between prepared MHCNs and CMs provided the enhanced stability, where the loss amount (7.0 mg g−1) was dramatically reduced in comparison with that of CMs adsorbed MHCNs (17.9 mg g−1) and MNs (18.7 mg g−1). The resultant CM-MHCNs showed high adsorption ability, rapid binding kinetics, good selectivity and reusability. Consequently, two novel potential anti-osteoporosis compounds: isopimpinellin and xanthotoxol were successfully discovered from Common Cnidium Fruit, which were confirmed by pharmacodynamic experiments. Their possible membrane receptors were further investigated by molecular docking and western blot analysis. These researches on CM-MHCNs offer a deep insight into green design of stable biomaterials and show their promising prospect in the discovery of drug leads, thus promoting the development of traditional herb medicines.