DNA as a nutrient novel role for bacterial competence gene homologs
DNA as a nutrient novel role for bacterial competence gene homologs
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DOI：
10.1128/jb.183.21.6288-6293.2001
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发表时间：
2001-11-01
影响因子：
3.2
通讯作者：
Kolter, R
中科院分区：
文献类型：
--
作者：
Finkel, SE;Kolter, R
细胞外DNA的摄取和稳定维持，即遗传转化，被普遍认为是微生物进化的主要力量。我们在这里表明，胞外DNA，无论是同种的还是异种的，也可以作为支持微生物生长的唯一碳和能量来源。不能消耗DNA的突变体在静止阶段的竞争中会遭受严重的适应性损失。在大肠杆菌中，DNA作为营养物质的使用依赖于参与流感嗜血杆菌和淋球菌自然遗传能力和转化的蛋白质的同源。这些大肠杆菌基因的同源基因存在于变形杆菌伽玛亚类的许多成员中，这表明DNA的消耗机制在进化过程中可能被广泛保守。
The uptake and stable maintenance of extracellular DNA, genetic transformation, is universally recognized as a major force in microbial evolution. We show here that extracellular DNA, both homospecific and heterospecific, can also serve as the sole source of carbon and energy supporting microbial growth. Mutants unable to consume DNA suffer a significant loss of fitness during stationary-phase competition. In Escherichia coli, the use of DNA as a nutrient depends on homologs of proteins involved in natural genetic competence and transformation in Haemophilus influenzae and Neisseria gonorrhoeae. Homologs of these E. coli genes are present in many members of the gamma subclass of Proteobacteria, suggesting that the mechanisms for consumption of DNA may have been widely conserved during evolution.