Solvent-Induced Assembly of Microbial Protein Nanowires into Superstructured Bundles
Solvent-Induced Assembly of Microbial Protein Nanowires into Superstructured Bundles
复制标题
溶剂诱导微生物蛋白质纳米线组装成超结构束
DOI：
10.1021/acs.biomac.0c01790
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发表时间：
2021
影响因子：
6.2
通讯作者：
Nonnenmann, Stephen S.
中科院分区：
文献类型：
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作者：
Sun, Yun-Lu;Montz, Brian J.;Selhorst, Ryan;Tang, Hai-Yan;Zhu, Jiaxin;Nevin, Kelly P.;Woodard, Trevor L.;Ribbe, Alexander E.;Russell, Thomas P.;Nonnenmann, Stephen S.
基于蛋白质的电子生物材料由于其对环境友好的生产和提纯，是传统金属和半导体材料的一种有吸引力的替代品。然而，阻碍这些材料进一步发展的主要挑战包括(1)与在有机溶剂中处理蛋白质有关的限制，(2)难以形成具有多长度尺度控制的高阶结构或支架。本论文解决了这两个挑战，导致了一维束的形成，由从微生物硫磺还原菌和大肠杆菌中获得的导电蛋白质纳米线组成。从常见的有机溶剂(如己烷、环己烷和DMF)中处理这些生物纳米线，可以生产出由清晰可见的菌毛组成的多长度尺度结构。透射电子显微镜显示了长达10μm的捆绑蛋白纳米线的惊人图像，宽度从50-500 nm(代表数十到数百条纳米线的组装)。导电原子力显微镜证实，在它们的束状状态下，存在明显的纳米线导电性。这些结果极大地扩展了制造基于蛋白质纳米线的电子设备架构的各种阵列的可能性。
Protein-based electronic biomaterials represent an attractive alternative to traditional metallic and semiconductor materials due to their environmentally benign production and purification. However, major challenges hindering further development of these materials include (1) limitations associated with processing proteins in organic solvents and (2) difficulties in forming higher-order structures or scaffolds with multilength scale control. This paper addresses both challenges, resulting in the formation of one-dimensional bundles composed of electrically conductive protein nanowires harvested from the microbesGeobacter sulfurreducensandEscherichia coli. Processing these bionanowires from common organic solvents, such as hexane, cyclohexane, and DMF, enabled the production of multilength scale structures composed of distinctly visible pili. Transmission electron microscopy revealed striking images of bundled protein nanowires up to 10 μm in length and with widths ranging from 50–500 nm (representing assembly of tens to hundreds of nanowires). Conductive atomic force microscopy confirmed the presence of an appreciable nanowire conductivity in their bundled state. These results greatly expand the possibilities for fabricating a diverse array of protein nanowire-based electronic device architectures.