The influence of rutin on the extracellular matrix in streptozotocin-induced diabetic rat kidney
The influence of rutin on the extracellular matrix in streptozotocin-induced diabetic rat kidney
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DOI：
10.1211/jpp.58.8.0010
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发表时间：
2006-08-01
影响因子：
3.3
通讯作者：
Prince, P. Stanely Mainzen
中科院分区：
文献类型：
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作者：
Kamalakkannan, N.;Prince, P. Stanely Mainzen
我们以前报道过，芦丁给药链脲佐菌素（STZ）诱导的糖尿病大鼠降低血糖和增加血浆胰岛素水平。在这项研究中，我们研究了芦丁对STZ诱导的糖尿病大鼠肾脏基质重塑的作用。对雄性白化病Wistar大鼠腹腔注射STZ（50 mg/kg）诱导实验性糖尿病。正常大鼠和STZ诱导的糖尿病大鼠连续45天口服芸香苷（100 mg kg-1），观察其对羟脯氨酸和胶原含量以及基质金属蛋白酶（MIMPs）活性的影响。我们还研究了肾脏中金属蛋白酶组织抑制剂（TIMPs）的水平。STZ诱导的糖尿病对照组大鼠肾脏羟脯氨酸和胶原含量增加，MMPs活性降低，TIMPs水平升高。在STZ诱导的糖尿病大鼠中，这些变化被芦丁治疗积极调制，从而保护肾脏。在正常大鼠与芦丁治疗，没有研究的参数显着改变。结果表明，芦丁通过影响基质金属蛋白酶的表达，对STZ诱导的大鼠肾脏损伤具有保护作用。观察到的效果是由于芦丁降低血糖水平。
We previously reported that rutin administration to streptozotocin (STZ)-induced diabetic rats decreased plasma glucose and increased plasma insulin levels. In this study, we have examined the role of rutin on matrix remodelling in the kidney of STZ-induced diabetic rats. STZ was administered intraperitoneally (50 mg kg(-1)) to male albino Wistar rats to induce experimental diabetes. Rutin (100 mg kg-1) was orally administered to normal and STZ-induced diabetic rats for a period of 45 days and its influence on the content of hydroxyproline and collagen and on the activity of matrix metalloproteinases (MIMPs) were studied. We have also studied the levels of tissue inhibitors of metalloproteinases (TIMPs) in the kidney. STZ-induced diabetic control rats showed increased content of hydroxyproline and collagen, decreased activity of MMPs and increased levels of TIMPs in the kidney. These changes were positively modulated by rutin treatment in STZ-induced diabetic rats, thereby protecting the kidney. In normal rats treated with rutin, none of the parameters studied were significantly altered. From the results obtained, we could conclude that rutin influences MMPs and effectively protects kidney against STZ-induced damage in rats. The effects observed are due to the reduction of plasma glucose levels by rutin.