Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay--a comparative study.
Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay--a comparative study.
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DOI：
10.1186/1748-717x-7-1
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发表时间：
2012-01-03
期刊：
影响因子：
--
通讯作者：
Langguth P
中科院分区：
文献类型：
--
作者：
Buch K;Peters T;Nawroth T;Sänger M;Schmidberger H;Langguth P
为了研究照射后癌细胞的增殖和存活测定，基于活细胞将黄色水溶性四唑盐还原为紫色水不溶性甲染料的多重MTT测定从单点法修改为增殖测定。该测定可使用多孔板在短时间内对大量样品进行，测定可使用酶标仪半自动进行。存活率是本试验中计算的参数，通过数学方法确定。在对照组和照射组中的指数生长被证明是多重MTT测定法的适用性的潜在基础。在两种设置中（包括照射前后的细胞平板接种）证明了与克隆形成存活测定的等效性，但其缺点如耗时。本实验选用A549、LN229和F98三种细胞株，研究其原理和通用性。
For studying proliferation and determination of survival of cancer cells after irradiation, the multiple MTT assay, based on the reduction of a yellow water soluble tetrazolium salt to a purple water insoluble formazan dye by living cells was modified from a single-point towards a proliferation assay. This assay can be performed with a large number of samples in short time using multi-well-plates, assays can be performed semi-automatically with a microplate reader. Survival, the calculated parameter in this assay, is determined mathematically. Exponential growth in both control and irradiated groups was proven as the underlying basis of the applicability of the multiple MTT assay. The equivalence to a clonogenic survival assay with its disadvantages such as time consumption was proven in two setups including plating of cells before and after irradiation. Three cell lines (A 549, LN 229 and F 98) were included in the experiment to study its principal and general applicability.