Mechanical stimulation improves tissue-engineered human skeletal muscle
Mechanical stimulation improves tissue-engineered human skeletal muscle
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DOI：
10.1152/ajpcell.00595.2001
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发表时间：
2002-11-01
影响因子：
5.5
通讯作者：
Vandenburgh, HH
中科院分区：
文献类型：
--
作者：
Powell, CA;Smiley, BL;Vandenburgh, HH
人类生物人工肌肉（HBAM）是通过将肌肉细胞悬浮在胶原/MATRIGEL中，在含有末端附着位点的硅胶模具中铸造，并允许细胞分化8至16天来进行组织工程化的。所得到的HBAM是骨骼肌的代表，因为它们含有平行阵列的有丝分裂后肌纤维，然而，它们在许多其他形态学特征上不同。设计改进的HBAM，即，更类似于体内，我们开发了机械细胞刺激器（MCS）硬件，以将类似体内的力直接施加到工程组织。一个敏感的力传感器连接到HBAM测量实时，内部产生的，以及外部施加的，力。肌细胞在形成过程中产生越来越多的内力，这与细胞骨架解聚剂是一致的。重复拉伸/放松8天，增加了HBAM弹性2至3倍，平均肌纤维直径12%，肌纤维面积百分比40%。该系统允许改进的骨骼肌类似物的工程以及非破坏性的方法来确定所得到的组织的被动力和粘弹性。
Human bioartificial muscles (HBAMs) are tissue engineered by suspending muscle cells in collagen/MATRIGEL, casting in a silicone mold containing end attachment sites, and allowing the cells to differentiate for 8 to 16 days. The resulting HBAMs are representative of skeletal muscle in that they contain parallel arrays of postmitotic myofibers; however, they differ in many other morphological characteristics. To engineer improved HBAMs, i.e., more in vivo-like, we developed Mechanical Cell Stimulator (MCS) hardware to apply in vivo-like forces directly to the engineered tissue. A sensitive force transducer attached to the HBAM measured real-time, internally generated, as well as externally applied, forces. The muscle cells generated increasing internal forces during formation which were inhibitable with a cytoskeleton depolymerizer. Repetitive stretch/relaxation for 8 days increased the HBAM elasticity two- to threefold, mean myofiber diameter 12%, and myofiber area percent 40%. This system allows engineering of improved skeletal muscle analogs as well as a nondestructive method to determine passive force and viscoelastic properties of the resulting tissue.