Further characterisation of a thromboembolic model of stroke in the rat
Further characterisation of a thromboembolic model of stroke in the rat
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DOI：
10.1016/s0006-8993(00)03331-x
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发表时间：
2001-03-23
期刊：
影响因子：
2.9
通讯作者：
Menon, DK
中科院分区：
文献类型：
--
作者：
Beech, JS;Williams, SCR;Menon, DK
我们用磁共振成像（MRI）技术观察了大鼠血栓栓塞性中风模型。通过使用梯度回波方法和T2* 图的产生，通过询问组织氧合状态，绘制与新形成血栓的大脑中动脉闭塞（MCAo）相关的急性灌注不足的后果。最终梗死面积随后在缺血后24小时通过2，3，5-氯化三苯基四氮唑（TTC）染色的组织学进行评估。动物显示梗死体积为178.7 +/- 84.2 mm（3）（平均值+/-S.D.）具有较大的变异系数（47%）和数值范围（85.6-265.5 mm（3））。这种变异性为我们提供了一个机会，以评估早期影像学观察和最终梗死面积之间的关系。对于MCA区域中心的单个大脑切片，MCAo后1和2小时T2* 降低的面积与最终病变面积高度相关（斯皮尔曼等级相关，r=0.98）。P
We have used magnetic resonance imaging (MRI) techniques to characterise a rat model of thromboembolic stroke. The consequences of acute perfusion deficit associated with a middle cerebral artery occlusion (MCAo) by a newly formed thrombus was mapped by interrogation of the tissue oxygenation status using gradient echo methods and production of T2* maps. Final infarct size was subsequently assessed at 24-h post-ischaemia by histology with 2,3,5-triphenyltetrazolium chloride (TTC) staining. Animals displayed an infarct volume of 178.7 +/- 84.2 mm(3) (mean +/-S.D.) with a large coefficient of variation (47%) and range of values (85.6-265.5 mm(3)). This variability provided us with an opportunity to assess the relationships between early imaging observations and eventual infarct size. For a single cerebral slice, at the centre of the MCA territory, a relationship between the area of reduced T2* at 1 and 2 h post MCAo correlated highly with final lesion area (Spearman rank correlation, r=0.98. P