High-angle triple-axis specimen holder for three-dimensional diffraction contrast imaging in transmission electron microscopy
High-angle triple-axis specimen holder for three-dimensional diffraction contrast imaging in transmission electron microscopy
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DOI：
10.1016/j.ultramic.2011.03.021
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发表时间：
2011-07-01
期刊：
影响因子：
2.2
通讯作者：
Midgley, P. A.
中科院分区：
文献类型：
--
作者：
Hata, S.;Miyazaki, H.;Midgley, P. A.
电子断层扫描需要宽角度范围的倾斜角，以进行可靠的三维（3D）重建。尽管样品架在商业上可用于断层摄影，但它们具有若干限制，包括最多仅在一个或两个轴上倾斜的能力，例如倾斜-旋转。对于非晶样品，图像对比度仅取决于质量和厚度，单倾斜保持器适用于大多数断层图像采集。另一方面，对于图像对比度强烈依赖于衍射条件的晶体材料，目前市售的断层摄影支架是不够的，因为它们缺乏在整个倾斜范围内保持恒定衍射条件所需的所有三个正交轴上的倾斜能力。我们已经开发了一种高角度三轴（HATA）层析成像标本保持器，能够在其他（正交）水平和垂直轴的倾斜能力的主要水平轴的高角度倾斜。这允许用户调整样品倾斜，以在层析成像系列的整个倾斜范围内获得所需的衍射条件。为了证明它的能力，我们已经使用了这个三轴层析保持器与双轴倾斜系列（试样旋转90度的系列之间的异位），以获得断层重建的位错排列在塑性变形奥氏体钢箔。(C)2011 Elsevier B. V.保留所有权利。
Electron tomography requires a wide angular range of specimen-tilt for a reliable three-dimensional (3D) reconstruction. Although specimen holders are commercially available for tomography, they have several limitations, including tilting capability in only one or two axes at most, e.g. tilt-rotate. For amorphous specimens, the image contrast depends on mass and thickness only and the single-tilt holder is adequate for most tomographic image acquisitions. On the other hand, for crystalline materials where image contrast is strongly dependent on diffraction conditions, current commercially available tomography holders are inadequate, because they lack tilt capability in all three orthogonal axes needed to maintain a constant diffraction condition over the whole tilt range. We have developed a high-angle triple-axis (HATA) tomography specimen holder capable of high-angle tilting for the primary horizontal axis with tilting capability in the other (orthogonal) horizontal and vertical axes. This allows the user to trim the specimen tilt to obtain the desired diffraction condition over the whole tilt range of the tomography series. To demonstrate its capabilities, we have used this triple-axis tomography holder with a dual-axis tilt series (the specimen was rotated by 90 degrees ex-situ between series) to obtain tomographic reconstructions of dislocation arrangements in plastically deformed austenitic steel foils. (C) 2011 Elsevier B.V. All rights reserved.