A LARGE CELL-ADHESIVE SCATTER FACTOR SECRETED BY HUMAN GASTRIC-CARCINOMA CELLS
A LARGE CELL-ADHESIVE SCATTER FACTOR SECRETED BY HUMAN GASTRIC-CARCINOMA CELLS
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DOI：
10.1073/pnas.90.24.11767
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发表时间：
1993-12-15
影响因子：
11.1
通讯作者：
UMEDA, M
中科院分区：
文献类型：
--
作者：
MIYAZAKI, K;KIKKAWA, Y;UMEDA, M
人胃癌细胞系STKM-1分泌一种大蛋白，其诱导大鼠肝上皮细胞系（BRL）在单层培养中分散成不连续的单个细胞。从STKM-1细胞的无血清条件培养基中纯化该细胞散射因子，发现其由140、150和160 kDa的三个二硫键连接的亚基组成。140-kDa肽含有与层粘连蛋白B2 t链同源的氨基酸序列。天然蛋白质的表观分子量>1000 kDa，pI为5.0。除了细胞分散活性外，纯化的蛋白质还刺激BRL细胞与基质的附着及其迁移。已观察到对各种细胞系的类似作用，包括非致瘤性上皮细胞系、内皮细胞系和成纤维细胞系以及人癌细胞系。人鳞状细胞癌和胃癌细胞系以及非致瘤性上皮细胞和内皮细胞系分泌类似的细胞散射活性。这些结果表明，蛋白质，命名为“ladsin”，可能是一种细胞外基质蛋白，调节细胞-细胞和细胞-底物相互作用和细胞迁移。
Human gastric carcinoma cell line STKM-1 secretes a large protein that induces scattering of a rat liver epithelial cell line (BRL) into disconnected individual cells in monolayer culture. This cell-scattering factor was purified from serum-free conditioned medium of STKM-1 cells and found to be composed of three disulfide-linked subunits of 140, 150, and 160 kDa. The 140-kDa peptide contains an amino acid sequence homologous to that of the laminin B2t chain. The native protein has an apparent molecular mass of >1000 kDa and a pI of 5.0. In addition to the cell-scattering activity, the purified protein stimulates attachment of BRL cells to substrate and their migration. Similar effects have been observed toward various cell lines, including nontumorigenic epithelial, endothelial, and fibroblastic cell lines and human cancer cell lines. Similar cell-scattering activity was secreted by human squamous carcinoma and gastric carcinoma cell lines and nontumorigenic epithelial and endothelial cell lines. These results indicate that the protein, named ''ladsin,'' is probably an extracellular matrix protein that regulates cell-cell and cell-substrate interactions and cell migration.