Methylobacterium oxalidis sp. nov., isolated from leaves of Oxalis corniculata.
Methylobacterium oxalidis sp. nov., isolated from leaves of Oxalis corniculata.
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DOI：
10.1099/ijs.0.033019-0
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发表时间：
2012-07
影响因子：
2.8
通讯作者：
A. Tani;N. Sahin;K. Kimbara
中科院分区：
文献类型：
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作者：
A. Tani;N. Sahin;K. Kimbara
从牛膝草（Oxalis corniculata）叶片中分离到一株粉红色兼性甲基营养细菌35a(T)。菌株35a(T)细胞为革兰氏反应阴性、可运动、不形成孢子的杆状细胞。菌株35a(T)与Methylobacterium iners 5317S-33(T)（96.7%）、Methylobacterium soli YIM 48816（96.6%）和Methylobacterium jeotgali S2R03-9(T)（96.3%）的16S rRNA基因序列相似性最高。甲基杆菌属的16S rRNA基因序列与其他已知种类型菌株的相似性均在96%以下。主要细胞脂肪酸为C(18:1)ω7c、C（18:0）和C（16:0）。DNA-DNA杂交实验、cpn60基因序列分析、脂肪酸谱分析、全细胞MALDI-TOF/MS谱图分析以及生理生化测试的结果表明，菌株35a(T)与其最近的系统发育邻居存在基因型和表型分化。因此，菌株35a(T)被认为代表了甲基细菌属中的一个新种，因此建议将其命名为甲基细菌草酸杆菌sp. 11。型应变为35a(T) (=DSM 24028（T)=NBRC 107715(T)）。
A pink-pigmented, facultatively methylotrophic bacterium, strain 35a(T), was isolated from the leaves of Oxalis corniculata. Cells of strain 35a(T) were Gram-reaction-negative, motile, non-spore-forming rods. The highest 16S rRNA gene pairwise sequence similarities for strain 35a(T) were found with the strains of Methylobacterium iners 5317S-33(T) (96.7%), 'Methylobacterium soli' YIM 48816 (96.6%) and Methylobacterium jeotgali S2R03-9(T) (96.3%). 16S rRNA gene sequence similarities with the type strains of all other recognized species of the genus Methylobacterium were below 96%. Major cellular fatty acids were C(18:1)ω7c, C(18:0) and C(16:0). The results of DNA-DNA hybridization experiments, analysis of cpn60 gene sequences, fatty acid profiles, whole-cell MALDI-TOF/MS spectral pattern analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 35a(T) from its nearest phylogenetic neighbours. Strain 35a(T) is therefore considered to represent a novel species within the genus Methylobacterium, for which the name Methylobacterium oxalidis sp. nov. is proposed. The type strain is 35a(T) (=DSM 24028(T)=NBRC 107715(T)).