Selective uptake of immunoglobulins by the proximal intestine of suckling rats.
Selective uptake of immunoglobulins by the proximal intestine of suckling rats.
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乳鼠近端肠选择性摄取免疫球蛋白。
DOI：
10.1152/ajplegacy.1972.223.6.1286
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
D. Mackenzie
中科院分区：
文献类型：
--
作者：
D. Mackenzie
* * * * * * * * * * * * * * *。哺乳大鼠近端肠对免疫球蛋白的选择性摄取。点。[j] .中国生物医学工程学报，2009(6):1286-1295。1972.采用放射性碘化（1251和1311）羊IgGl、IgG2和大鼠IgG，研究了哺乳大鼠肠道对免疫球蛋白的选择性吸收机制。IgG2的选择性吸收发生在将igg1和IgG2的混合物通过结扎从远端肠分离出来的近端肠中。当混合物放置在远端肠中时，这两种蛋白质的吸收较小且相等。近端肠外翻囊对IgG2和大鼠IgG的体外摄取随其在培养基中的浓度而变化，随着浓度的每一次增加，摄取的增加比例较小。大鼠IgG抑制IgG2的摄取。IgGl的摄取低于IgG2和大鼠IgG。远端囊泡对所有三种蛋白的摄取是相似的，并且与它们在培养基中的浓度成正比。虽然不排除其他可能性，但结果与近端肠上皮细胞上特异性受体位点的存在是一致的，这些受体位点与大鼠IgG、>、羊IgGz、>、羊IgGr等免疫球蛋白有亲和力。免疫球蛋白的吸收;免疫球蛋白吸附;免疫球蛋白特异性受体；远端肠免疫球蛋白摄取；大鼠免疫球蛋白;在许多哺乳动物物种中，蛋白质，特别是IgG类免疫球蛋白，从母体血液循环转移到幼畜血液循环(5)。完成这一过程的方式因物种而异。因此，在男性中，IgG通过胎盘在子宫内转移（14-16）；兔和豚鼠通过卵黄囊内胚层从子宫腔吸收母源IgG (8,26,28)；大鼠和小鼠在子宫内通过卵黄囊内胚层获得一些免疫球蛋白（6,18），但也在出生后通过选择性地从乳汁中吸收IgG获得一些免疫球蛋白（7,18,23,33 -35）；反刍动物的初乳含有非常高浓度的IgG(36,37)，在出生后第1天被新生儿吸收（30,37）。所有这些蛋白质转移系统是否存在共同的潜在机制尚不清楚，因为在任何一个系统中，这一过程都知之甚少。从Brambell和他的同事的工作中得知（4,5），在大鼠中同源IgG和来自许多其他物种的IgG通过肠道转移。免疫球蛋白Fc片段的未知性质可能决定了是否有任何par-
~ &ACKENZIE, DD s. Selective uptake of immunoglobdins by the proximal intestine of suckling rats. Am. J. Physiol. 223 (6): 1286-1295. 1972.-Radioiodinated(1251 and 1311) sheep IgGl and IgG2 and rat IgG were used to study the mechanism of selective absorption of immunoglobulins by the intestine of suckling rats. Selective absorption of IgG2 occurred when a mixture of IgG 1 and IgG2 was placed in the proximal intestine isolated in vivo from the distal intestine by a ligature. Absorption of both proteins was smaller and equal when the mixture was placed in the distal intestine. The uptake in vitro of IgG2 and rat IgG by everted sacs of proximal intestine varied with their concentration in the medium so that with each increase in concentration there was a proportionally smaller increase in uptake. Rat IgG inhibited the uptake of IgG2. The uptake of IgGl was smaller than that of either IgG2 or rat IgG. Uptake of all three pro-teins by distal sacs was similar and proportional to their concentration in the medium. The results, although not excluding other possibilities, are consistent with the presence of specific receptor sites on the epithelial cells of the proximal intestine which have an affinity for immunoglobulins in the order rat IgG> sheep IgGz> sheep IgGr. immunoglobulin absorption; immunoglobulin adsorption; specific receptors for immunoglobulin; immunoglobulin uptake by distal intestine; rat IgG; sheep IgGTRANSFER OF PROTEINS, particularly immunoglobulins of the IgG class, from the maternal circulation to that of the young occurs in many mammalian species (5). The manner by which this is accomplished varies markedly between species. Thus, in man IgG is transferred in utero across the placenta(14-16); rabbits and guinea pigs absorb IgG of maternal origin through the yolk sac endoderm from the uterine cavity (8, 26, 28); rats and mice acquire some immunoglobulins in utero by way of the yolk sac endoderm(6, 18), but also receive some after birth by selectively absorbing IgG from the milk (7, 18, 23, 33-35); the colostrum of ruminants contains very high concentrations of IgG (36, 37), which is absorbed by the newborn during the 1st day after birth (30, 37). Whether there is an underlying mechanism common to all these systems of protein transfer is not known, since in any one system the process is poorly understood. It is known from the work of Brambell and his colleagues (4, 5) that in the rat homologous IgG and IgG from a number of other species are transferred across the intestine. Unknown properties of the Fc fragment of the mmunoglobulin probably determine whether any par-