HYDROXYL-RADICAL-INDUCED IRON-CATALYSED DEGRADATION OF 2-DEOXYRIBOSE - QUANTITATIVE-DETERMINATION OF MALONDIALDEHYDE
HYDROXYL-RADICAL-INDUCED IRON-CATALYSED DEGRADATION OF 2-DEOXYRIBOSE - QUANTITATIVE-DETERMINATION OF MALONDIALDEHYDE
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DOI：
10.1042/bj2520649
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发表时间：
1988-06-15
影响因子：
4.1
通讯作者：
ESTERBAUER, H
中科院分区：
文献类型：
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作者：
CHEESEMAN, KH;BEAVIS, A;ESTERBAUER, H
用两个羟基自由基生成系统研究了2-脱氧核糖降解为硫代巴比妥酸反应物质：(i)一个定义的。-放射性溶解法和（ii）在磷酸缓冲液中与FeSO4孵育。在每种情况下，硫代巴比妥酸反应物质都可以用丙二醛来解释，用高效液相色谱法测定游离丙二醛。在放射分解系统中，如果向样品中加入铁离子，则游离丙二醛在辐照后会大量增加。有人提出，这是由于铁离子催化了放射性分解产生的H2O2形成羟基自由基，并刺激了中间脱氧核糖降解产物的分解。提出了脱氧核糖降解过程中丙二醛形成的机理。
The degradation of 2-deoxyribose to thiobarbituric acid-reactive material was investigated with two hydroxyl-radical-generating systems: (i) a defined .gamma.-radiolysis method and (ii) incubation with FeSO4 in phosphage buffer. In each case the thiobarbituric acid-reactive material can be accounted for by malondialdehyde, as measured by an h.p.l.c. method for free malondialdehyde. In the radiolysis system there is a large post-irradiaton increase in free malondialdehyde if iron ions are added to the samples. It is proposed that this is due to iron ions catalysing the formation of hydroxyl radicals from radiolytically generated H2O2 as well as stimulating the breakdown of an intermediate deoxyribose degradation product. A mechanism for the formation of malondialdehyde during deoxyribose degradation is proposed.