Kinetoplast DNA replication: mechanistic differences between Trypanosoma brucei and Crithidia fasciculata.
Kinetoplast DNA replication: mechanistic differences between Trypanosoma brucei and Crithidia fasciculata.
复制标题
动力学DNA复制：Brucei和Crithidia fasciculata之间的机械差异。
DOI：
10.1083/jcb.126.3.631
复制
发表时间：
1994-08
影响因子：
7.8
通讯作者：
Englund, P T
中科院分区：
文献类型：
--
作者：
Ferguson, M L;Torri, A F;Perez-Morga, D;Ward, D C;Englund, P T
动质体DNA是锥虫的线粒体DNA，是一个包含数千个小环和几十个大环的网络。我们比较了动基体DNA复制在布氏锥虫和Crithidia fasciculata使用荧光原位杂交和电子显微镜孤立的网络。一个不同之处是在位置的maxicircles在原位。In C. fasciculata，maxicircles集中在离散的焦点嵌入动基体盘;在复制过程中，焦点的数量增加，但仍然分散在整个磁盘。与此相反，T.布氏菌大环通常充满整个盘。与C中的不同。fasciculata、T.在复制后，布氏大环高度集中在动基体的中心区域;然后在分离期间，它们重新分布在整个子动基体中。T. brucei和C. fasciculata也不同的新合成的小环的网络连接的模式。In C.在束状体中，已知微环附着在两个对足位点，但随后发现微环均匀地分布在网络外围周围，这可能是由于动基体盘和负责微环合成和附着的两种蛋白质复合物的相对运动。于T.在布氏杆菌中，微环似乎附着在两个对足位点，但随后保持集中在这两个区域。因此，在T.布鲁塞。
Kinetoplast DNA, the mitochondrial DNA of trypanosomatid parasites, is a network containing several thousand minicircles and a few dozen maxicircles. We compared kinetoplast DNA replication in Trypanosoma brucei and Crithidia fasciculata using fluorescence in situ hybridization and electron microscopy of isolated networks. One difference is in the location of maxicircles in situ. In C. fasciculata, maxicircles are concentrated in discrete foci embedded in the kinetoplast disk; during replication the foci increase in number but remain scattered throughout the disk. In contrast, T. brucei maxicircles generally fill the entire disk. Unlike those in C. fasciculata, T. brucei maxicircles become highly concentrated in the central region of the kinetoplast after replication; then during segregation they redistribute throughout the daughter kinetoplasts. T. brucei and C. fasciculata also differ in the pattern of attachment of newly synthesized minicircles to the network. In C. fasciculata it was known that minicircles are attached at two antipodal sites but subsequently are found uniformly distributed around the network periphery, possibly due to a relative movement of the kinetoplast disk and two protein complexes responsible for minicircle synthesis and attachment. In T. brucei, minicircles appear to be attached at two antipodal sites but then remain concentrated in these two regions. Therefore, the relative movement of the kinetoplast and the two protein complexes may not occur in T. brucei.