RAPID, SPECIFIC, DEFINED SUBSTRATE TECHNOLOGY FOR THE SIMULTANEOUS DETECTION OF TOTAL COLIFORMS AND ESCHERICHIA-COLI
RAPID, SPECIFIC, DEFINED SUBSTRATE TECHNOLOGY FOR THE SIMULTANEOUS DETECTION OF TOTAL COLIFORMS AND ESCHERICHIA-COLI
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DOI：
10.1002/tox.2540030511
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发表时间：
1988-11-01
期刊：
影响因子：
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通讯作者：
SMITH, DB
中科院分区：
文献类型：
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作者：
EDBERG, SC;ALLEN, MJ;SMITH, DB
自动分析Colilert（AC）检测是对特定底物技术的改良，旨在从混合物中计数特定靶微生物。所有成分都以粉末形式存在于管或容器中。AC测试执行起来很简单-将测量量的水添加到试管（用于最可能的数量测试）或容器[存在/不存在（P/A）测试]中。孵育后，黄色的发展是特定的总大肠菌群。同一试管中的荧光对大肠杆菌是特异性的。不需要进行确证或完整的试验。对美国所有水源和水处理进行了一次全国性评价。AC试验与多管发酵（定量）和P/A（定性）标准方法程序进行了比较。测试方案是美国环境保护署环境监测和支持实验室的方案，用于确定方法之间的等效性。定量比较表明，AC测试的灵敏度略高于MTF。五个地点中有两个更精确，其他三个是相等的。对于定性P/A，AC和标准方法分析的一致性为94%。通过传代培养一种总大肠菌群或E.大肠杆菌阳性。AC测试易于使用，可以由大型和小型公用事业公司执行，并且比当前的方法更便宜。能同时特异性地计数大肠菌群和大肠杆菌。大肠杆菌而不需要确认试验。
The Autoanalysis Colilert (AC) test is a modification of the defined substrate technology designed to enumerate specific target microbe(s) from a mixture. All ingredients are present in the tube or vessel in powdered form. The AC test is simple to perform—a measured amount of water is added to test tubes (for the most probable number test) or vessel [Presence/Absence (P/A) test]. After incubation, the development of a yellow color is specific for total coliforms. Fluorescence in the same tube(s) is specific forEscherichia coli.No confirmatory or completed tests need be performed. A national United States evaluation covering all sources and processing of water was conducted. The AC test was compared to multiple tube fermentation (quantitative) and P/A (qualitative)Standard Methodsprocedures. Testing protocol was that of the U.S. Environmental Protection Agency's Environmental Monitoring and Support Laboratory for establishing equivalency between methods. The quantitative comparison showed that the AC test was slightly more sensitive than MTF. For two of five sites it was more precise; for the other three it was equal. For the qualitative P/A the AC andStandard Methodsanalysis agreed 94%. Specificity of the AC method was established by subculturing a species of total coliform orE. colifrom positives. The AC test was easy to use, can be performed by large and small utilities, and is less expensive than current methods. It can simultaneously, specifically enumerate total coliforms andE. coliwithout the need for confirmatory tests.