Antibiotic susceptibility and genotypic characterization of Haemophilus influenzae strains isolated from nasopharyngeal specimens from children in day-care centers in eastern France.
Antibiotic susceptibility and genotypic characterization of Haemophilus influenzae strains isolated from nasopharyngeal specimens from children in day-care centers in eastern France.
复制标题
从法国东部日托中心儿童鼻咽标本中分离出的流感嗜血杆菌菌株的抗生素敏感性和基因型特征。
DOI：
10.1046/j.1469-0691.2000.00151.x
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Estavoyer Jm
中科院分区：
文献类型：
--
作者：
D. Talon;Joel Leroy;M. Dupont;Xavier Bertrand;F. Mermet;M. Thouverez;Estavoyer Jm
目的
确定日托中心幼儿流感嗜血杆菌的总体携带率、对各类抗生素的耐药频率以及各种耐药表型分离株之间的克隆关系。
方法
获得鼻咽（NP）标本，并在含有杆菌肽的巧克力琼脂上培养。对所有分离株进行抗生素敏感性试验和血清分型。应用脉冲场凝胶电泳SmaI酶切技术研究氨苄青霉素敏感菌株和产β-内酰胺酶菌株的遗传多态性。
结果
在596份NP分泌物培养物中，152份（25.5%）为H阳性。流感。64株（42.1%）产β-内酰胺酶，2株（1.3%）耐氨苄西林但不产β-内酰胺酶。我们无法对150个分离株进行血清分型;一个分离株属于荚膜血清型e，一个属于血清型f。在76株随机分离的菌株中鉴定出46种主要的DNA模式。产β-内酰胺酶的菌株比氨苄青霉素敏感的菌株更易出现EP（P < 10（-4））。与EP的频率显着降低日托中心参加了不到20名儿童比参加了超过20名儿童（P = 0.020）。
结论
由于产生β-内酰胺酶而产生的耐药性在一些日托中心传播，主要是通过人与人之间的传播，但也可能通过菌株之间大质粒的配偶转移。日托中心的规模可能会影响传播的风险。
OBJECTIVE
To determine the overall carriage rate for Haemophilus influenzae in young children in day-care centers, the frequency of resistance to various classes of antibiotic, and the clonal relationship between isolates of the various resistant phenotypes.
METHODS
Nasopharyngeal (NP) specimens were obtained and cultured on chocolate agar with bacitracin. Antibiotic susceptibility testing and serotyping were performed for all isolates. The genetic polymorphism of ampicillin-susceptible and beta-lactamase-producing isolates was studied by pulsed-field gel electrophoresis using SmaI.
RESULTS
Of the 596 NP secretion cultures, 152 (25.5%) were positive for H. influenzae. Sixty-four (42.1%) isolates produced beta-lactamase and two (1.3%) were ampicillin resistant but did not produce beta-lactamase. We were unable to serotype 150 isolates; one isolate belonged to capsular serotype e and one to serotype f. Forty-six major DNA patterns were identified among 76 randomized isolates. beta-lactamase producing isolates more frequently showed EP than ampicillin-susceptible isolates P < 10(-4). The frequency of isolates with EP was significantly lower in day-care centers attended by less than 20 children than in those attended by more than 20 children (P = 0.020).
CONCLUSIONS
Resistance due to beta-lactamase production has disseminated in some day-care centers, mostly by person-to-person spread but also via the possible conjugal transfer of large plasmids between strains. The size of day-care centers may affect the risk of transmission.
影响因子：
6.4
作者：
FADEN, H;HARABUCHI, Y;HONG, JJ
通讯作者：
HONG, JJ
DOI：
--
发表时间：
1990
期刊：
The Pediatric infectious disease journal
影响因子：
--
作者：
Faden,H;Stanievich,J;Brodsky,L;Bernstein,J;Ogra,PL
通讯作者：
Ogra,PL
易患中耳炎和非易患中耳炎儿童鼻咽部细菌菌群的微生态。
影响因子：
1.4
作者：
Bernstein,JM;Faden,HF;Dryja,DM;Wactawski-Wende,J
通讯作者：
Wactawski-Wende,J