Changes in progesterone receptor isoforms content in the rat brain during the oestrous cycle and after oestradiol and progesterone treatments
Changes in progesterone receptor isoforms content in the rat brain during the oestrous cycle and after oestradiol and progesterone treatments
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DOI：
10.1046/j.1365-2826.2003.01088.x
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发表时间：
2003-10-01
影响因子：
3.2
通讯作者：
Camacho-Arroyo, I
中科院分区：
文献类型：
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作者：
Guerra-Araiza, C;Villamar-Cruz, O;Camacho-Arroyo, I
用免疫印迹法研究了雌二醇和孕酮对去卵巢大鼠和正常大鼠发情周期中孕酮受体(PR)亚型含量的影响。去卵巢大鼠下丘脑和视前区PR-A和PR-B含量明显升高，孕酮处理后3h下丘脑PR-A和PR-B亚型含量显著降低，而视前区PR-A和PR-B含量无明显变化。在海马区，雌二醇处理12h后，只有PR-A含量显著增加，而孕酮显著降低。在额叶皮质，NO处理显著改变PR亚型含量。在发情周期中，下丘脑PR亚型含量在发情间期最低，而在视前区，两种PR亚型含量均在发情间期最高。在发情周期中，我们没有观察到海马区PR亚型含量的变化。这些结果表明，PR亚型的表达受性类固醇激素的区域特异性调节。
We studied the effects of oestradiol and progesterone on progesterone receptor (PR) isoform content in the brain of ovariectomized rats and in intact rats during the oestrous cycle by Western blot analysis. In the hypothalamus and the preoptic area of ovariectomized rats, PR-A and PR-B content was increased by oestradiol, whereas progesterone significantly diminished the content of both PR isoforms after 3 h of treatment in the hypothalamus, but not in the preoptic area. In the hippocampus, only PR-A content was significantly increased by oestradiol while progesterone significantly diminished it after 12 h of treatment. In the frontal cortex, no treatment significantly modified PR isoform content. During the oestrous cycle, the lowest content of PR isoforms in the hypothalamus was observed on diestrus day and, by contrast, in the preoptic area, the highest content of both PR isoforms was observed on diestrus day. We observed no changes in PR isoform content in the hippocampus during the oestrous cycle. These results indicate that the expression of PR isoforms is differentially regulated by sex steroid hormones in a regionally specific manner.