Rapid de novo generation of defective interfering RNA by cucumber necrosis virus mutants that do not express the 20-kDa nonstructural protein.
Rapid de novo generation of defective interfering RNA by cucumber necrosis virus mutants that do not express the 20-kDa nonstructural protein.
复制标题
通过不表达 20-kDa 非结构蛋白的黄瓜坏死病毒突变体快速从头生成有缺陷的干扰 RNA。
DOI：
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发表时间：
1991
影响因子：
11.1
通讯作者：
D. Rochon
中科院分区：
文献类型：
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作者：
D. Rochon
一般认为，高感染倍数（moi）的连续传代是产生缺陷干扰（DI）粒子的必要条件。高水平的DI rna被发现与黄瓜坏死病毒（CNV）实验室培养引发的持续感染有关。两种合成的CNV转录物，通过位点定向诱变获得的高感染性CNV cDNA克隆，不表达CNV 20 kda非结构蛋白，发现在转录接种的植物中无需连续高通量传代即可从头产生高水平的减症状DI rna。这种DI rna的重新生成在由野生型转录物引发的感染中不会发生，直到至少8个连续的高速率传代。观察到CNV非结构蛋白突变体快速生成DI RNA从头开始，可能为RNA病毒中DI颗粒形成的机制提供了见解。
It is generally believed that serial passage at high multiplicity of infection (moi) is required for the generation of defective interfering (DI) particles. High levels of DI RNAs are found associated with persistent infections initiated with laboratory cultures of cucumber necrosis virus (CNV). Two synthetic CNV transcripts that were derived through site-directed mutagenesis of a highly infectious CNV cDNA clone and that do not express the CNV 20-kDa nonstructural protein were found to generate high levels of symptom-attenuating DI RNAs de novo without serial high-moi passage in transcript-inoculated plants. Such de novo generation of DI RNAs did not occur in infections initiated with wild-type transcript until at least eight serial high-moi passages. The observation that a CNV nonstructural protein mutant rapidly generates DI RNA de novo may provide insight into mechanisms that underly DI particle formation in RNA viruses in general.