Developmental changes in ciliary motility on choroid plexus epithelial cells during the perinatal period.
Developmental changes in ciliary motility on choroid plexus epithelial cells during the perinatal period.
复制标题
围产期脉络丛上皮细胞纤毛运动的发育变化。
DOI：
10.1002/cm.21132
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发表时间：
2013
期刊：
影响因子：
2.9
通讯作者：
Sen Takeda
中科院分区：
文献类型：
--
作者：
Yuta Nonami;Keishi Narita;Hideki Nakamura;Takafumi Inoue;Sen Takeda
纤毛在各种发育和生理活动中起着至关重要的作用。以前，我们报道脉络丛上皮细胞(CPECs)有多个不活动的9+0纤毛，但在新生期纤毛表现出瞬时的运动和不同的轴线排列。这些特征使这些纤毛独一无二，因为它们不适合传统的初级或活动纤毛类别，它们的生理作用仍然难以捉摸。为了解决这一问题，我们研究了CPECs在发育过程中的纤毛运动，特别是在胚胎期。在胎儿侧脑室脉络丛中，具有活动纤毛的细胞比例及其搏动频率随着时间的推移而增加。纤毛运动曲线在出生当天达到高峰，在出生后两周内逐渐下降。纤毛运动的动态变化与Dnahc11表达的变化相关。我们以前证明过，P2的纤毛运动不足以产生可检测到的液体流动；因此，CPEC纤毛在发育过程中的任何时候都不产生液体流动。综上所述，我们的结果表明，在围产期，CPEC纤毛可能存在一种时间调节的独特功能。©2013 Wiley期刊，Inc.
Cilia have crucial roles in various developmental and physiological events. Previously, we reported that choroid plexus epithelial cells (CPECs) have multiple, nonmotile 9+0 cilia, but the cilia exhibit transient motility with variable axonemal arrangements in the neonatal period. These features make these cilia unique, as they do not fit in to the traditional categories of primary or motile cilia, and their physiological roles remain elusive. To address this issue, we studied ciliary motility on CPECs through development, with particular interest in the embryonic period. In the fetal choroid plexus of the lateral ventricles, the proportion of cells with motile cilia and their beat frequency increased over time. The ciliary motility profiles peaked near the day of birth, and gradually declined in the two weeks thereafter. The dynamic changes in ciliary motility correlated with changes inDnahc11expression. We demonstrated previously that the ciliary motility at P2 was insufficient to produce detectable fluid flow; thus it appears that CPEC cilia do not produce fluid flow at any point during development. Together, our results suggest that a temporally regulated, unique function of CPEC cilia may exist during the perinatal period. © 2013 Wiley Periodicals, Inc.