Identification of novel alternatively spliced pyruvate carboxylase mRNAs with divergent 5'-untranslated regions which are expressed in a tissue-specific manner
Identification of novel alternatively spliced pyruvate carboxylase mRNAs with divergent 5'-untranslated regions which are expressed in a tissue-specific manner
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DOI：
10.1006/bbrc.1996.0958
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发表时间：
1996-06-25
影响因子：
3.1
通讯作者：
Wallace, JC
中科院分区：
文献类型：
--
作者：
Jitrapakdee, S;Walker, ME;Wallace, JC
我们使用cDNA 5'末端快速扩增-聚合酶链反应（RACE-PCR）鉴定并表征了大鼠和人丙酮酸羧化酶的多种mRNA转录物[EC 6.4.1.1]。已经在肝、肾、脑和脂肪组织中鉴定了大鼠丙酮酸羧化酶cDNA的五种替代形式，并且这些以组织特异性方式表达。还在肝脏中鉴定了人丙酮酸羧化酶cDNA的两种替代形式。这些丙酮酸羧化酶cDNA具有共同的编码区，但在它们的5'非翻译区（5' UTR）中不同，这表明它们是通过初级转录物的选择性剪接产生的。限制性内切酶消化的大鼠基因组DNA的Southern印迹分析显示，丙酮酸羧化酶是由一个单拷贝基因编码。(C)出版社：Academic Press，Inc.
We have identified and characterized multiple mRNA transcripts of rat and human pyruvate carboxylases [EC 6.4.1.1] using rapid amplification of cDNA 5' ends-polymerase chain reaction (RACE-PCR). Five alternative forms of rat pyruvate carboxylase cDNAs have been identified in liver, kidney, brain, and adipose tissue and these are expressed in a tissue-specific manner. Two alternative forms of human pyruvate carboxylase cDNA have also been identified in liver. These pyruvate carboxylase cDNAs have a common coding region but differ in their 5' untranslated regions (5'UTRs), suggesting that they are generated by alternative splicing of the primary transcript. Southern blot analysis of restriction enzyme digested rat genomic DNA revealed that pyruvate carboxylase is encoded by a single copy gene. (C) 1996 Academic Press, Inc.