Involvement of a PadR regulator PrhP on virulence of Ralstonia solanacearum by controlling detoxification of phenolic acids and type III secretion system
Involvement of a PadR regulator PrhP on virulence of Ralstonia solanacearum by controlling detoxification of phenolic acids and type III secretion system
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PadR 调节剂 PrhP 通过控制酚酸解毒和 III 型分泌系统参与青枯雷尔斯顿菌的毒力
DOI：
10.1111/mpp.12854
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发表时间：
2019-08
影响因子：
4.9
通讯作者：
Ohnishi Kouhei
中科院分区：
文献类型：
--
作者：
Zhang Yong;Zhang Weiqi;Han Liangliang;Li Jing;Shi Xiaojun;Hikichi Yasufumi;Ohnishi Kouhei
摘要 青枯雷尔斯顿菌可以代谢阿魏酸（FA）和水杨酸（SA）这两种代表性的酚酸，以保护其免受酚酸的毒性。在这里，我们从基因角度证明了一种新型酚酸脱羧酶调节剂（PadR）样调节剂 PrhP 作为青枯菌中 SA 和 FA 解毒的正调节剂。尽管降解SA和FA的能力增强了青枯菌对宿主植物的感染过程，但除SA和FA的降解外，PrhP对感染过程也有很大贡献。我们的生长测定、启动子活性测定、RNA-seq 和 qRT-PCR 的结果表明，PrhP 在青枯菌的毒力中发挥多种作用：（1）正向调节 SA 和 FA 降解基因的表达； (2) 在体外和植物体内正向调节编码 III 型分泌系统 (T3SS) 和 III 型效应子的基因的表达； (3)正向调节许多毒力相关基因的表达，如鞭毛、IV型菌毛和细胞壁降解酶等； (4)对于植物体内的广泛增殖很重要。 T3SS 是许多病原菌中重要的致病性决定因素之一，PrhP 通过关键调节因子 HrpB 介导，但通过一些新的青枯菌中的 HrpB 途径，正向调节其表达。这是第一篇关于PadR调节剂调节T3SS的报道，它可以提高我们对青枯菌中PadR调节剂的各种生物学功能和T3SS复杂调节途径的理解。
Summary Ralstonia solanacearum can metabolize ferulic acid (FA) and salicylic acid (SA), two representative phenolic acids, to protect it from toxicity of phenolic acids. Here, we genetically demonstrated a novel phenolic acid decarboxylase regulator (PadR)‐like regulator PrhP as a positive regulator on detoxification of SA and FA in R. solanacearum. Although the ability to degrade SA and FA enhances the infection process of R. solanacearum toward host plants, PrhP greatly contributes to the infection process besides degradation of SA and FA. Our results from the growth assay, promoter activity assay, RNA‐seq and qRT‐PCR revealed that PrhP plays multiple roles in the virulence of R. solanacearum: (1) positively regulates expression of genes for degradation of SA and FA; (2) positively regulates expression of genes encoding type III secretion system (T3SS) and type III effectors both in vitro and in planta; (3) positively regulates expression of many virulence‐related genes, such as the flagella, type IV pili and cell wall degradation enzymes; and (4) is important for the extensive proliferation in planta. The T3SS is one of the essential pathogenicity determinants in many pathogenic bacteria, and PrhP positively regulates its expression mediated with the key regulator HrpB but through some novel pathway to HrpB in R. solanacearum. This is the first report on PadR regulators to regulate the T3SS and it could improve our understanding of the various biological functions of PadR regulators and the complex regulatory pathway on T3SS in R. solanacearum.
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影响因子：
4.4
作者：
J. Tans-Kersten;Y. Guan;C. Allen
通讯作者：
J. Tans-Kersten;Y. Guan;C. Allen
影响因子：
7.4
作者：
J. Smith-Becker;E. Marois;E. Huguet;S. Midland;J. Sims;N. Keen
通讯作者：
J. Smith-Becker;E. Marois;E. Huguet;S. Midland;J. Sims;N. Keen
影响因子：
5.2
作者：
Wu D;Ding W;Zhang Y;Liu X;Yang L
通讯作者：
Yang L
影响因子：
2.1
作者：
Yong Zhang;A. Kiba;Y. Hikichi;K. Ohnishi
通讯作者：
Yong Zhang;A. Kiba;Y. Hikichi;K. Ohnishi
影响因子：
5.6
作者：
Hikichi Y;Mori Y;Ishikawa S;Hayashi K;Ohnishi K;Kiba A;Kai K
通讯作者：
Kai K