Improving membrane voltage measurements using FRET with new fluorescent proteins
Improving membrane voltage measurements using FRET with new fluorescent proteins
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DOI：
10.1038/nmeth.1235
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发表时间：
2008-08-01
期刊：
影响因子：
48
通讯作者：
Miyawaki, Atsushi
中科院分区：
文献类型：
--
作者：
Tsutsui, Hidekazu;Karasawa, Satoshi;Miyawaki, Atsushi
我们使用两种新的珊瑚荧光蛋白作为荧光共振能量转移(FRET)供体和受体来开发一种名为美人鱼的电压传感器，该传感器每100 mV的发射比变化类似于40%，允许直接观察培养的可兴奋细胞的电活动。值得注意的是，美人鱼在温暖(类似于33摄氏度)的温度下具有快速的开关动力学，并且可以报告与动作电位相当的电压尖峰。
We used two new coral fluorescent proteins as fluorescence resonance energy transfer (FRET) donor and acceptor to develop a voltage sensor, named Mermaid, that displays similar to 40% changes in emission ratio per 100 mV, allowing for direct visualization of electrical activities in cultured excitable cells. Notably, Mermaid has fast on-off kinetics at warm (similar to 33 degrees C) temperatures and can report voltage spikes comparable to action potentials.