Identification of Diverse Alphacoronaviruses and Genomic Characterization of a Novel Severe Acute Respiratory Syndrome-Like Coronavirus from Bats in China
Identification of Diverse Alphacoronaviruses and Genomic Characterization of a Novel Severe Acute Respiratory Syndrome-Like Coronavirus from Bats in China
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DOI：
10.1128/jvi.00631-14
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发表时间：
2014-06-01
影响因子：
5.4
通讯作者：
Tu, Changchun
中科院分区：
文献类型：
--
作者：
He, Biao;Zhang, Yuzhen;Tu, Changchun
尽管在中国、欧洲和非洲的蝙蝠中发现了许多严重急性呼吸综合征样冠状病毒（SARS样冠状病毒），但大多数在介导受体结合并决定宿主谱的受体结合域（RBD）中具有与人类/果子狸SARS冠状病毒明显不同的遗传组织，导致它们不能引起人类感染，使它们不太可能是人类/果子狸SARS冠状病毒的祖病毒。本文对云南省四个县收集的268只蝙蝠直肠拭子进行了病毒宏基因组分析，确定了数百个与甲型冠状病毒和乙型冠状病毒相关的序列。基于RNA依赖RNA聚合酶基因保守区域的系统发育分析显示，甲型冠状病毒具有多样性，与以往报道的病毒有明显差异。对来自宝山的新型SARS样冠状病毒（LYRa11）的全基因组分析显示，该病毒全长29,805个核苷酸（nt），有13个开放阅读框（orf），与人类/果子狸SARS样冠状病毒和最近报道的SARS样冠状病毒Rs3367具有91%的核苷酸同源性，与其他蝙蝠SARS样冠状病毒具有89%的同源性。值得注意的是，它与SARS冠状病毒S基因和Rs3367的序列一致性最高，特别是在RBD地区。抗原分析表明，LYRa11的S1结构域可被SARS恢复期人血清有效识别，提示LYRa11是一种与SARS冠状病毒抗原相近的新型病毒。重组分析表明，LYRa11可能是亲本谱系的重组后代，亲本谱系已进化成许多蝙蝠sars样冠状病毒。
Although many severe acute respiratory syndrome-like coronaviruses (SARS-like CoVs) have been identified in bats in China, Europe, and Africa, most have a genetic organization significantly distinct from human/civet SARS CoVs in the receptor-binding domain (RBD), which mediates receptor binding and determines the host spectrum, resulting in their failure to cause human infections and making them unlikely progenitors of human/civet SARS CoVs. Here, a viral metagenomic analysis of 268 bat rectal swabs collected from four counties in Yunnan Province has identified hundreds of sequences relating to alpha-and betacoronaviruses. Phylogenetic analysis based on a conserved region of the RNA-dependent RNA polymerase gene revealed that alphacoronaviruses had diversities with some obvious differences from those reported previously. Full genomic analysis of a new SARS-like CoV from Baoshan (LYRa11) showed that it was 29,805 nucleotides (nt) in length with 13 open reading frames (ORFs), sharing 91% nucleotide identity with human/civet SARS CoVs and the most recently reported SARS-like CoV Rs3367, while sharing 89% with other bat SARS-like CoVs. Notably, it showed the highest sequence identity with the S gene of SARS CoVs and Rs3367, especially in the RBD region. Antigenic analysis showed that the S1 domain of LYRa11 could be efficiently recognized by SARS-convalescent human serum, indicating that LYRa11 is a novel virus antigenically close to SARS CoV. Recombination analyses indicate that LYRa11 is likely a recombinant descended from parental lineages that had evolved into a number of bat SARS-like CoVs.