Integrated processes for production of cellulosic and hemicellulosic biobutanol from sweet sorghum bagasse using autohydrolysis
Integrated processes for production of cellulosic and hemicellulosic biobutanol from sweet sorghum bagasse using autohydrolysis
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DOI：
10.1016/j.indcrop.2019.111918
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发表时间：
2020-03
影响因子：
5.9
通讯作者：
F. Khalili;H. Amiri
中科院分区：
文献类型：
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作者：
F. Khalili;H. Amiri
基于自动水解、酶水解和 ABE 发酵的整合，评估了从甜高粱甘蔗渣 (SSB) 生产半纤维素和纤维素丁醇的不同工艺替代方案。使用商业半纤维素酶混合物对通过单阶段或两阶段自动水解获得的液体进行后水解并通过丙酮丁醇梭菌发酵。在自水解的每个阶段获得的预处理固体也可用于通过使用商业纤维素酶混合物的单独水解和发酵（SHF）或同时糖化和发酵（SSF）来生产ABE。两阶段自动水解（150°C 30分钟；120°C 60分钟）随后酶促后水解产生6.6g/L糖并发酵至4.6g/L ABE。另一方面，预处理的 SSB 进行酶水解和 ABE 发酵，得到 4.7-5.4g/L ABE。在此过程中，每公斤SSB可生产32克半纤维素和85克纤维素ABE。此外，还根据 SSB 生产 ABE 的总产量对其他 13 种工艺替代方案进行了比较。在最紧凑的工艺中，四个酶水解和发酵阶段被组合成一个名为“同时共糖化和发酵（SCSF）”的阶段，产生 21−22g ABE/kg SSB。
Different process alternatives were assessed for production of hemicellulosic and cellulosic butanol from sweet sorghum bagasse (SSB) based on integration of autohydrolysis, enzymatic hydrolysis, and ABE fermentation. The liquor obtained through single or two-stage autohydrolysis was subjected to post-hydrolysis using a commercial hemicellulases blend and fermentation byClostridium acetobutylicum. The pretreated solids obtained at each stage of autohydrolysis were also used for ABE production through separated hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF) using a commercial cellulase blend. The two-stage autohydrolysis (at 150 °C for 30 min; 120 °C for 60 min) followed by enzymatic post-hydrolysis resulted in 6.6 g/L sugar and fermented to 4.6 g/L ABE. On the other hand, enzymatic hydrolysis and ABE fermentation of the pretreated SSB led to 4.7–5.4 g/L ABE. In this process, 32 g hemicellulosic and 85 g cellulosic ABE were produced from each kg SSB. Besides, other thirteen process alternatives were compared based on the overall yield of ABE production from SSB. In the most compact process, the four enzymatic hydrolysis and fermentation stages were combined into a single stage named “simultaneous co-saccharification and fermentation (SCSF)" leading to 21−22 g ABE/kg SSB.