Tire debris organic extract affects Xenopus development
Tire debris organic extract affects Xenopus development
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DOI：
10.1016/j.envint.2007.01.007
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发表时间：
2007-07-01
影响因子：
11.8
通讯作者：
Camatini, Marina
中科院分区：
文献类型：
--
作者：
Mantecca, Paride;Gualtieri, Maurizio;Camatini, Marina
轮胎碎片（TD）及其有机成分被确定为 PM10 大气和水污染的主要来源。由于关于 TD 有机成分的胚胎毒性作用的数据很少，因此使用青蛙胚胎致畸试验 - 爪蟾 (FETAX) 并结合存活幼虫的组织病理学筛选来评估轮胎碎片有机提取物 (TDOE) 的致死和致畸潜力。从第8阶段到第47阶段，非洲爪蟾胚胎暴露于浓度为50、80、100、120和140 mg/L的TDOE。结果显示，50 mg/L TDOE 为不可观察效应浓度 (NOEC)。 TDOE 死亡率在 80 mg/L 时显着高于对照，但不会随着浓度的升高而进一步增加。观察到畸形幼虫的百分比具有良好的浓度响应，并且从 80 mg/L 开始，这些百分比显着高于对照。因此，概率分析得出 TC50 为 144.6 mg/L。在 120 和 140 mg/L 浓度下，许多幼虫呈多形。观察到的最常见的改变是肠道卷曲异常、小眼球、单侧无眼球和眼睛缩小。组织学检查主要发现双视网膜、视网膜神经细胞层卷曲、晶状体改变等眼部畸形。此外，肝脏和轴向肌肉组织出现严重的空泡形成和坏死。这些结果有力地支持了 TDOE 对非洲爪蟾来说是一种强大的致畸剂的假设。 (c) 2007 Elsevier Ltd. 保留所有权利。
Tire debris (TD) and its organic components were identified as a main source of PM10 atmospheric and water pollution. Because few data are available on the embryotoxic effects of TD organic components, the lethal and teratogenic potential of tire debris organic extract (TDOE) was evaluated using the frog embryo teratogenesis assay-Xenopus (FETAX), coupled with a histopathological screening of the survived larvae. From stage 8 to stage 47,Xenopus laevis embryos were exposed to TDOE at concentrations of 50, 80, 100, 120 and 140 mg/L. The results showed 50 mg/L TDOE to be the non-observable effect concentration (NOEC). TDOE mortality at 80 mg/L was significantly higher than the control, but did not increase further with higher concentrations. A good concentration-response was observed for percentages of malformed larva and from 80 mg/L on these percentages were significantly higher than the control. Therefore, probit analysis gave a 144.6 mg/L TC50. At 120 and 140 mg/L, many larvae were plurimalformed. The most frequent alterations observed were abnormal gut coiling, microphthalmia, monolateral anophthalmia, and narrowing eyes. The histological screening mainly revealed ocular malformations such as double retina, retina nervous cell layer coiling, and altered lens. Moreover severe vacuolisation and necrosis were scored in liver and axial musculature. These results strongly support the assumption that TDOE is a powerful teratogen for X. laevis. (c) 2007 Elsevier Ltd. All rights reserved.