Identification of novel noncoding transcripts in telomerase-negative yeast using RNA-seq.
Identification of novel noncoding transcripts in telomerase-negative yeast using RNA-seq.
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DOI：
10.1038/srep19376
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发表时间：
2016-01-20
影响因子：
4.6
通讯作者：
Zappulla DC
中科院分区：
文献类型：
--
作者：
Niederer RO;Papadopoulos N;Zappulla DC
端粒酶是一种核糖核蛋白，在大多数真核生物中维持线性染色体的末端。端粒酶活性的丧失导致端粒DNA缩短，最终导致特定的G2/M细胞周期停滞，称为衰老。在人类中，端粒缩短发生在衰老过程中，而端粒酶的不适当激活与大约90%的癌症有关。先前的研究已经确定了几类与衰老相关的衰老和癌症相关的非编码RNA（ncRNA），但ncRNA是否也参与酵母中短端粒诱导的衰老尚不清楚。在这里，我们报告了112个推定的新lncRNA在酵母酿酒酵母，其中41只在端粒酶阴性酵母表达。大约一半的lncRNA的表达与相邻基因的表达密切相关，这表明该子集可能影响相邻基因的转录。我们的研究结果揭示了一个新的潜在机制，在衰老和衰老后的幸存者酵母细胞的适应性变化。
Telomerase is a ribonucleoprotein that maintains the ends of linear chromosomes in most eukaryotes. Loss of telomerase activity results in shortening of telomeric DNA and eventually a specific G2/M cell-cycle arrest known as senescence. In humans, telomere shortening occurs during aging, while inappropriate activation of telomerase is associated with approximately 90% of cancers. Previous studies have identified several classes of noncoding RNAs (ncRNA) also associated with aging-related senescence and cancer, but whether ncRNAs are also involved in short-telomere-induced senescence in yeast is unknown. Here, we report 112 putative novel lncRNAs in the yeast Saccharomyces cerevisiae, 41 of which are only expressed in telomerase-negative yeast. Expression of approximately half of the lncRNAs is strongly correlated with that of adjacent genes, suggesting this subset may influence transcription of neighboring genes. Our results reveal a new potential mechanism governing adaptive changes in senescing and post-senescent survivor yeast cells.