NbLRK1, a lectin-like receptor kinase protein of Nicotiana benthamiana, interacts with Phytophthora infestans INF1 elicitin and mediates INF1-induced cell death
NbLRK1, a lectin-like receptor kinase protein of Nicotiana benthamiana, interacts with Phytophthora infestans INF1 elicitin and mediates INF1-induced cell death
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DOI：
10.1007/s00425-008-0797-y
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发表时间：
2008-11-01
期刊：
影响因子：
4.3
通讯作者：
Terauchi, Ryohei
中科院分区：
文献类型：
--
作者：
Kanzaki, Hiroyuki;Saitoh, Hiromasa;Terauchi, Ryohei
致病疫霉INF1诱导素引起本塞姆氏烟草的过敏反应(HR)(Kamoun等人，Plant Cell 10：1413-1425，1998)。为了鉴定与 INF1 相互作用的本塞姆氏烟草蛋白，我们进行了酵母双杂交筛选。该筛选结果分离出编码新型凝集素样受体激酶的基因 NbLRK1。 NbLRK1 通过其 VIb 激酶子域与 INF1 相互作用。纯化的 INF1 和 NbLRK1 蛋白也在体外相互作用。 N. Benthamiana 叶子的 INF1 处理诱导 NbLRK1 的自磷酸化。最重要的是，病毒诱导的 NbLRK1 基因沉默 (VIGS) 延迟了本塞姆氏烟草中 INF1 介导的 HR。这些数据表明 NbLRK1 是本塞姆氏烟草蛋白复合物的一个组成部分，可识别 INF1 激发子并转导 HR 信号。
Phytophthora infestans INF1 elicitin causes the hypersensitive response (HR) in Nicotiana benthamiana (Kamoun et al. in Plant Cell 10:1413-1425, 1998). To identify N. benthamiana proteins that interact with INF1, we carried out a yeast two-hybrid screen. This screen resulted in the isolation of a gene NbLRK1 coding for a novel lectin-like receptor kinase. NbLRK1 interacted with INF1 through its VIb kinase subdomain. Purified INF1 and NbLRK1 proteins also interacted in vitro. INF1 treatment of N. benthamiana leaves induced autophosphorylation of NbLRK1. Most importantly, virus-induced gene silencing (VIGS) of NbLRK1 delayed INF1-mediated HR in N. benthamiana. These data suggest that NbLRK1 is a component of the N. benthamiana protein complex that recognizes INF1 elicitor and transduces the HR signal.