Molecular analyses for identification of the Gracilariaceae (Rhodophyta) from the Asia–Pacific region

Molecular analyses for identification of the Gracilariaceae (Rhodophyta) from the Asia–Pacific region
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亚太地区江蓠科(红藻门)鉴定的分子分析

DOI:
10.1007/s13258-015-0306-1
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发表时间:
2015
期刊:
影响因子:
2.1
通讯作者:
M. Kim
M. Kim
中科院分区:
生物学4区
文献类型:
--
作者:
M. Yang;M. Kim

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龙舌兰科(Gracilariaceae)含有重要的经济海藻,由于其高度的物种多样性和物种限度的不确定性,被认为是物种鉴定的一个困难类群。到目前为止,亚太地区对龙舌兰科的分子研究还不足以确定物种的分布和多样性。为了更好地了解物种边界和物种多样性,我们分析了作为DNA条码标记的线粒体COI和来自亚太地区的样本的质体rbcL基因。共有22种被确认为一个不同的实体,其中包括5个未确认的物种和2个新的分布记录。COI序列数据比RBCL数据表现出更多的变异,使物种的区分变得清晰。COI种内差异范围为0-2.6%(除4.4%外),种间差异范围为2.8-15.7%。RbcL数据显示,种间差异在1.1~14.8%之间,分为两大支系,即感官江豚(Gracilaria Sensu Lato)和龙须菜(Gracilariopsis)。这项区域研究使我们获得了台湾江豚和澳大利亚水龙两个物种的地理分布信息,并首次报道了它们在日本冲绳的存在。龙须菜属植物数据库的建立对加快物种多样性鉴定具有重要意义。
The family Gracilariaceae contains economically important seaweeds and is well known as a difficult group for the species identification due to its high species diversity and uncertainty of species limits. To date, molecular studies of the Gracilariaceae in the Asia–Pacific region have been insufficient to determine the distribution and diversity of species. To acquire a better understanding of the species boundary and species diversity, we analyzed mitochondrial COI as DNA barcoding marker and plastid rbcL genes for specimens from the Asia–Pacific region. A total of 22 species has been recognized as a distinct entity including five unidentified and two new distribution records. COI sequence data showed more variation than rbcL data, enabling the clear discrimination of species. The range of intra- and inter-specific divergence for COI was 0–2.6 % (except 4.4 %) and 2.8–15.7 %, respectively. RbcL data showed the interspecific divergences ranged from 1.1 to 14.8 % and divided into two large clades, such as Gracilaria sensu lato and Gracilariopsis. The regional study allowed us to get the information of the geographic distribution of two species, Gracilariopsis chiangii from Taiwan and Hydropuntia perplexa from Australia, and reported for the first time their presence in Okinawa, Japan. The database of the Gracilariaceae has potential to speed up the identification of species diversity.
Gracilaria parvispora 是韩国和日本 G. bursa-pastoris 的正确名称
DOI: --
发表时间: 2008
期刊: Taxon 57
影响因子: --
作者:
Kim;Kim;Terada;Yamg and Boo
通讯作者: Yamg and Boo
DOI: 10.1093/molbev/msm092
发表时间: 2007-08-01
影响因子: 10.7
作者:
Tamura, Koichiro;Dudley, Joel;Kumar, Sudhir
通讯作者: Kumar, Sudhir