Effect of maternal protein restriction during pregnancy and postweaning high-fat feeding on diet-induced thermogenesis in adult mouse offspring
Effect of maternal protein restriction during pregnancy and postweaning high-fat feeding on diet-induced thermogenesis in adult mouse offspring
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DOI：
10.1007/s00394-014-0657-4
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发表时间：
2014-10-01
影响因子：
5
通讯作者：
Cagampang, Felino R.
中科院分区：
文献类型：
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作者：
Sellayah, Dyan;Dib, Lea;Cagampang, Felino R.
产前营养不良以及断奶后高脂肪饮食喂养会导致成年后代肥胖。在这项研究中，我们研究了饮食诱导的生热作用是否会因这种营养不匹配而改变。在整个怀孕和哺乳期，雌性 MF-1 小鼠被喂食正常蛋白质（NP，18% 酪蛋白）或蛋白质限制（PR，9% 酪蛋白）饮食。断奶后，两组雄性后代均饲喂高脂饮食（HF；45% kcal 脂肪）或标准饲料（C，7% kcal 脂肪），形成 NP/C、NP/HF、PR/C 和 PR/HF 成年后代组（每组 n = 7-11）。PR/C 和 NP/C 后代在 30 周龄时体重相似。与普通饲料喂养的后代相比，断奶后 HF 喂养导致 NP/HF 后代显着增重 (P < 0.01)，但 PR/HF 后代则不然。然而，与 NP/HF 组相比，PR/HF 后代表现出更大的肥胖（P < 0.01）。与 NP/C 小鼠相比，NP/HF 后代的能量消耗增加，肩胛间棕色脂肪组织 (iBAT) 中解偶联蛋白 1 和 β 3 肾上腺素受体 mRNA 表达增加（均 P < 0.01）。 PR/HF 和 PR/C 后代之间在能量消耗和 iBAT 基因表达方面没有观察到这种差异。这些数据表明，怀孕和哺乳期母亲饮食与后代断奶后饮食之间的不匹配，会减弱饮食诱导的 iBAT 生热作用，导致成年期肥胖的发生。
Prenatal undernutrition followed by postweaning feeding of a high-fat diet results in obesity in the adult offspring. In this study, we investigated whether diet-induced thermogenesis is altered as a result of such nutritional mismatch.Female MF-1 mice were fed a normal protein (NP, 18 % casein) or a protein-restricted (PR, 9 % casein) diet throughout pregnancy and lactation. After weaning, male offspring of both groups were fed either a high-fat diet (HF; 45 % kcal fat) or standard chow (C, 7 % kcal fat) to generate the NP/C, NP/HF, PR/C and PR/HF adult offspring groups (n = 7-11 per group).PR/C and NP/C offspring have similar body weights at 30 weeks of age. Postweaning HF feeding resulted in significantly heavier NP/HF offspring (P < 0.01), but not in PR/HF offspring, compared with their chow-fed counterparts. However, the PR/HF offspring exhibited greater adiposity (P < 0.01) v the NP/HF group. The NP/HF offspring had increased energy expenditure and increased mRNA expression of uncoupling protein-1 and beta-3 adrenergic receptor in the interscapular brown adipose tissue (iBAT) compared with the NP/C mice (both at P < 0.01). No such differences in energy expenditure and iBAT gene expression were observed between the PR/HF and PR/C offspring.These data suggest that a mismatch between maternal diet during pregnancy and lactation, and the postweaning diet of the offspring, can attenuate diet-induced thermogenesis in the iBAT, resulting in the development of obesity in adulthood.