Conversion of methionine into homocysteic acid in heavily oxidized proteomics samples
Conversion of methionine into homocysteic acid in heavily oxidized proteomics samples
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DOI：
10.1002/rcm.4447
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发表时间：
2010-03-01
影响因子：
2
通讯作者：
Sharp, Joshua S.
中科院分区：
文献类型：
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作者：
Bern, Marshall;Saladino, Jessica;Sharp, Joshua S.
蛋白质氧化分析在生物化学的许多领域是必要的，包括羟基自由基表面作图，氧化应激测定和药物稳定性测试。质谱法是最常用于鉴定蛋白质氧化产物的工具之一，并且先前的研究已经尝试鉴定和表征通过质谱法检测到的每个氨基酸残基的所有主要氧化产物。在这篇文章中，我们提出的证据表明，在严重氧化的蛋白质样品，如那些产生的羟基自由基表面映射，甲硫氨酸的主要氧化产物是同型半胱氨酸。从蛋氨酸形成同型半胱氨酸是以前未识别的其他质谱分析，并具有重要的意义，氧化样品的分析，以及潜在的影响，蛋氨酸氧化的功能后果。版权所有（C）2010约翰威利父子有限公司
Analysis of protein oxidation is necessary in numerous areas of biochemistry, including hydroxyl radical surface mapping, oxidative stress assays, and pharmaceutical stability testing. Mass spectrometry is one of the tools most often used to identify protein oxidation products, and previous studies have attempted to identify and characterize all of the major oxidation products detected by mass spectrometry for each amino acid residue. In this note, we present evidence that in heavily oxidized protein samples, such as those produced by hydroxyl radical surface mapping, a major oxidation product of methionine is homocysteic acid. The formation of homocysteic acid from methionine was previously unrecognized in other mass spectrometric analyses, and has important implications for the analysis of oxidized samples, as well as potential implications as to the functional consequences of methionine oxidation. Copyright (C) 2010 John Wiley & Sons, Ltd.