Isolation and identification of an enterovirus 77 recovered from a refugee child from Kosovo, and characterization of the complete virus genome
Isolation and identification of an enterovirus 77 recovered from a refugee child from Kosovo, and characterization of the complete virus genome
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DOI：
10.1016/j.virusres.2003.11.006
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发表时间：
2004-02-01
期刊：
影响因子：
5
通讯作者：
Peigue-Lafeuille, H
中科院分区：
文献类型：
--
作者：
Bailly, JL;Cardoso, MC;Peigue-Lafeuille, H
本文报道了一株肠道病毒77型分离株的核苷酸全序列。从一名因沙门氏菌胃肠炎住院的4岁儿童的粪便中回收了命名为FR/CF 496 -99（法国/克莱蒙-费兰德496-1999）的病毒。通过基于编码VP 1衣壳蛋白的基因组序列的分子分型试验鉴定病毒。基于VP 1序列的系统发育分析表明，该儿童分离的肠道病毒与人类肠道病毒B种（HEV-B）中的病毒聚簇，与肠道病毒77的关系最密切。完整基因组的滑动窗口分析显示FR/CF 496 -99分离株的P3基因组区域与埃可病毒30原型株的P3基因组区域之间的总体核苷酸相似性>80%。基于部分3D（pol）序列的比较分析表明，FR/CF 496 -99病毒与来自不同血清型和不同地理区域的最近肠道病毒的关系比20世纪50年代收集的原型菌株更密切。这表明，在这种肠道病毒中，3D（pol）编码序列是最近的起源。(C)2003 Elsevier B. V.保留所有权利。
The complete nucleotide sequence of an enterovirus 77 isolate is reported. The virus designated FR/CF496-99 (France/Clermont-Ferrand 496-1999) was recovered from the feces of a 4-year-old child hospitalized for Salmonella gastroenteritis. The virus was identified by a molecular typing assay based on the genomic sequence encoding the VP1 capsid protein. The phylogenetic analysis based on the VP1 sequence demonstrated that the enterovirus isolated in the child clustered with viruses included in the human enterovirus B species (HEV-B) and was most closely related to enterovirus 77. A sliding window analysis of the complete genome showed an overall nucleotide similarity >80% between the P3 genomic region of the FR/CF496-99 isolate and that of the echovirus 30 prototype strain. A comparative analysis based on partial 3D(pol) sequences showed that the FR/CF496-99 virus was more closely related to recent enteroviruses from different serotypes and different geographical areas than to the prototype strains collected in the 1950s. This suggests that, in this enterovirus, the 3D(pol) encoding sequence is of recent origin. (C) 2003 Elsevier B.V. All rights reserved.