Characterization of Inflammatory Markers and Transcriptome Profiles of Differentially Activated Embryonic Stem Cell-Derived Microglia
Characterization of Inflammatory Markers and Transcriptome Profiles of Differentially Activated Embryonic Stem Cell-Derived Microglia
复制标题
DOI：
10.1002/glia.22979
复制
发表时间：
2016-06-01
期刊：
影响因子：
6.2
通讯作者：
Zimmer, Andreas
中科院分区：
文献类型：
--
作者：
Beins, Eva;Ulas, Thomas;Zimmer, Andreas
小胶质细胞是中枢神经系统的免疫细胞，是高度适应性的细胞，可以获得不同的促炎和抗炎激活状态，在中枢神经系统稳态和病理学中具有不同的功能。为了在体外研究小胶质细胞功能，通常使用原代小胶质细胞或永生化细胞系。这些细胞的替代品是胚胎干细胞衍生的小胶质细胞（ESdM）。此前已证明 ESdM 在表达谱和表面分子方面与原代小胶质细胞非常相似。在这项研究中，ESdM 和原代小胶质细胞用不同的炎症刺激剂进行处理，以分析它们采取不同激活状态的能力。使用定量实时 PCR、比较转录组学、ELISA 和流式细胞术，我们发现 ESdM 中可以诱导不同的激活状态，这与原代小胶质细胞中发现的激活状态相似。这些状态的特征是特定的炎症标记分子组和差异转录组特征。我们的结果表明，ESdM 是研究小胶质细胞功能和神经炎症机制的一种有价值的替代细胞模型。
Microglia, the immune cells of the CNS, are highly adaptive cells that can acquire different pro-and anti-inflammatory activation states with distinct functions in CNS homeostasis and pathologies. To study microglial function in vitro, primary microglia or immortalized cell lines are commonly used. An alternative to these cells are embryonic stem cell-derived microglia (ESdM). ESdM have previously been shown to be very similar to primary microglia in terms of expression profiles and surface molecules. In this study, ESdM and primary microglia were treated with different inflammatory stimulants to analyze their ability to adopt different activation states. Using quantitative real-time PCR, comparative transcriptomics, ELISA, and flow cytometry, we found that different activation states can be induced in ESdM, which are similar to those found in primary microglia. These states are characterized by specific sets of inflammatory marker molecules and differential transcriptome signatures. Our results show that ESdM are a valuable alternative cell model to study microglial functions and neuroinflammatory mechanisms.