Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol
Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol
复制标题
乙醇改性核桃蛋白-黄原胶复合胶粘剂的理化、结构及粘合性能
DOI：
10.1016/j.ijbiomac.2021.10.022
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发表时间：
2021-10-15
影响因子：
8.2
通讯作者：
Li, Shugang
中科院分区：
文献类型：
--
作者：
Lei, Yuqing;Gao, Sihai;Li, Shugang
低糖高蛋白胶粘剂具有广阔的市场应用前景，而天然植物蛋白粘结性较差，面临技术瓶颈。研究了不同浓度乙醇（0 ~ 80%）对核桃分离蛋白-黄原胶（WNPI-XG）复合胶粘剂粘附性能的影响。结果表明，经40%乙醇处理后的WNPI-XG的结合强度达到12.55 MPa，变性温度和表面疏水性分别提高到87.91和185.07，具有最佳的流变学和织构性能。适当浓度的乙醇（40%）没有改变WNPI-XG的分子量，但极大地增强了荧光强度，导致活性巯基、静电力、疏水相互作用、氢键和二硫键的含量发生变化。此外，该处理还促进了二级结构的构象转换，从i -sheet到α -helix，促进了蛋白质分子的完全展开。微观结构分析表明，经40%乙醇处理后，WNPI结构均匀，WNPI- xg胶粘剂表面平整光滑，与水分子结合更加紧密。通过分析乙醇处理对WNPI-XG粘附的影响，为蛋白质修饰奠定理论基础，为其开发利用提供良好的技术参考。
Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion. In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated. Results showed the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity increased to 87.91 and 185.07 respectively, displaying the best rheological and texture properties. It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds. Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from fl-sheet to alpha-helix, promoting the full unfolding of protein molecules. The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules. By analyzing the influence of ethanol treatment on adhesion of WNPI-XG, the research laid a theoretical foundation for protein modification, providing good technical references for its development and utilization.