Relationships between body temperatures and inflammation indicators under physiological and pathophysiological conditions in pigs exposed to systemic lipopolysaccharide and dietary deoxynivalenol
Relationships between body temperatures and inflammation indicators under physiological and pathophysiological conditions in pigs exposed to systemic lipopolysaccharide and dietary deoxynivalenol
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DOI：
10.1111/jpn.12684
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发表时间：
2018-02-01
影响因子：
2.7
通讯作者：
Daenicke, S.
中科院分区：
文献类型：
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作者：
Tesch, T.;Bannert, E.;Daenicke, S.
我们研究了健康猪和暴露于脂多糖 (LPS) 和/或脱氧雪腐镰刀菌烯醇 (DON) 的猪的直肠温度和腹腔内温度之间的关系的稳定性以及它们与炎症标志物（白细胞计数、犬尿氨酸与色氨酸比率（Kyn-Trp 比率）、肿瘤坏死因子 α (TNF-α)）的联系。Barrows (n = 44) 饲喂 4 周，或者DON 污染（4.59 mg DON/kg 饲料）或对照（CON）饮食，并配备腹内温度记录仪和多导管系统（肝门静脉、肝门静脉、颈静脉），便于输注 0.9% NaCl (CON) 或 LPS (7.5 μg/kg BW)，并每 15 分钟测量体温并采集白细胞样本。饮食和输注的组合创建了六组：CON_CONjug.-CONpor.、CON_CONjug.-CONpor.、CON_LPSjug.-CONpor.、DON_CONjug.-CONpor.、DON_CONjug.-LPSpor.、DON_LPSjug.-CONpor.。线性回归显示，在所有条件下，两个温度之间的关系并不均匀。与注射氯化钠的猪相比，直肠温度每升高 1 摄氏度，腹内温度升高 25%，尽管饮食和注射脂多糖的部位改变了这种差异的程度。炎症标志物仅在脂多糖影响下强烈存在，并且与体温显着相关。例如，临床上不明显的动物的白细胞计数仅与 DON 喂养的猪的核心温度显着相关。输注 LPS 的组，无论饮食和温度方法如何，总而言之，在临床上不显眼的猪中，身体核心温度和直肠温度之间的梯度是恒定的，但在后者的各种病理生理条件下，炎症标记物的测量似乎是一个有用的完成。
We studied the constancy of the relationship between rectal and intraabdominal temperature as well as their linkage to inflammatory markers (leucocyte counts, kynurenine-to-tryptophan ratio (Kyn-Trp ratio), tumour necrosis factor alpha (TNF-alpha) in healthy and in pigs exposed to lipopolysaccharide (LPS) and/or deoxynivalenol (DON). Barrows (n = 44) were fed 4 weeks either a DON-contaminated (4.59 mg DON/kg feed) or a control (CON) diet and equipped with an intraabdominal temperature logger and a multicatheter system (V.portae hepatis, V.lienalis, Vv.jugulares) facilitating infusion of 0.9% NaCl (CON) or LPS (7.5 mu g/kg BW) and simultaneous blood sampling. Body temperatures were measured and blood samples taken every 15 min for leucocyte counts, TNF-alpha and Kyn-Trp ratio. Combination of diet and infusion created six groups: CON_CONjug.-CONpor., CON_CONjug.-LPSpor., CON_LPSjug.-CONpor., DON_CONjug.-CONpor., DON_CONjug.-LPSpor., DON_LPSjug.-CONpor.. The relationship between both temperatures was not uniform for all conditions. Linear regression revealed that an intraabdominal increase per 1 degrees C increase in rectal temperature was similar to 25% higher in all LPS-infused pigs compared to NaCl-infusion, albeit diet and site of LPS infusion modified the magnitude of this difference. Inflammatory markers were only strongly present under LPS influence and showed a significant relationship with body temperatures. For example, leucocyte counts in clinically inconspicuous animals were only significantly correlated to core temperature in DON-fed pigs, but in all LPS-infused groups, irrespective of diet and temperature method. In conclusion, the gradient between body core and rectal temperature is constant in clinically inconspicuous pigs, but not under various pathophysiological conditions. In the latter, measurement of inflammatory markers seems to be a useful completion.