Integrin β1 is bound to galectin-1 in human trophoblast
Integrin β1 is bound to galectin-1 in human trophoblast
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DOI：
10.1093/jb/mvx061
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发表时间：
2018-01-01
影响因子：
2.7
通讯作者：
Vicovac, Ljiljana
中科院分区：
文献类型：
--
作者：
Bojic-Trbojevic, Zanka;Krivokuca, Milica Jovanovic;Vicovac, Ljiljana
糖结合蛋白-半乳糖凝集素与糖缀合物的相互作用被认为与各种生殖过程相关。半乳凝素-1（gal-1）是一种参与滋养层细胞侵袭的分子，其通过与细胞表面和/或细胞外基质糖蛋白相互作用来实现。研究了内源性gal-1和滋养层β（1）整合素相互作用的可能性，这两种整合素以前都显示与滋养层侵袭相关。共聚焦显微镜显示gal-1和β（1）整合素在分离的细胞滋养层细胞、HTR-8/SVneo绒毛外滋养层细胞系和JAr绒毛膜癌细胞的质膜定位重叠。免疫沉淀证实了gal-1与整合素β（1）的相互作用，但不与α（1）或α（5）整合素亚基。非变性电泳和亚细胞分级分离表明，在HTR-8/SVneo细胞的细胞内和质膜区室中，gal-1与β（1）整联蛋白相关。Gal-1/β（1）整联蛋白复合物对化学和酶处理敏感，表明碳水化合物依赖性相互作用。然而，通过qPCR和流式细胞术测定，siRNA下调gal-1对β（1）整联蛋白的水平或分布没有影响。这些结果表明，在滋养层细胞膜上，gal-1与β（1）整合素之间存在复杂的凝集素型相互作用，可能影响滋养层细胞的粘附、迁移和侵袭。
Interaction of sugar binding proteins-galectins, with glycoconjugates is considered relevant for various reproductive processes. Galectin-1 (gal-1) is a molecule involved in trophoblast cell invasion, which is accomplished through interaction with cell surface and/or extracellular matrix glycoproteins. A possibility of interaction of endogenous gal-1 and trophoblast beta(1) integrins, both previously shown relevant for trophoblast invasion, was investigated. Confocal microscopy showed overlap in gal-1 and beta(1) integrin localization at the plasma membrane of isolated cytotrophoblast, HTR-8/SVneo extravillous trophoblast cell line and JAr choriocarcinoma cells. Immunoprecipitation confirmed an interaction of gal-1 with integrin beta(1), but not with alpha(1) or alpha(5) integrin subunits. Nondenaturing electrophoresis and subcellular fractionation suggested association of gal-1 with beta(1) integrin in intracellular and plasma membrane compartments of HTR-8/SVneo cells. Gal-1/beta(1) integrin complex was sensitive to chemical and enzyme treatments, indicating carbohydrate dependent interaction. Down-regulation of gal-1 by siRNA, however, had no effect on level or distribution of beta(1) integrin, as determined by qPCR and flow cytometry. These results suggest complex lectin type interaction of gal-1 with beta(1) integrin at the trophoblast cell membrane, which could influence trophoblast cell adhesion, migration and invasion.