Developmental Expression of the Pluripotency Factor Sal-Like Protein 4 in the Monkey, Human and Mouse Testis: Restriction to Premeiotic Germ Cells
Developmental Expression of the Pluripotency Factor Sal-Like Protein 4 in the Monkey, Human and Mouse Testis: Restriction to Premeiotic Germ Cells
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DOI：
10.1159/000335031
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Behr, R.
中科院分区：
文献类型：
--
作者：
Eildermann, K.;Aeckerle, N.;Behr, R.
SALL4（sal 样蛋白 4）是一种多能性转录因子，在胚胎干 (ES) 细胞中高度表达，对于小鼠植入前发育至关重要。在成年小鼠器官中，Sall4 mRNA在睾丸和卵巢中高表达，而在其他器官中只有很少或没有表达。 SALL4 在人睾丸生殖细胞肿瘤中也有高表达。然而，目前还没有对哺乳动物睾丸发育过程中SALL4表达的详细分析。我们分析了非人灵长类 (NHP) 物种（普通狨猴 (Callithrix jacchus)）的 ES 细胞、植入前胚胎以及发育中和成年睾丸中的 SALL4 表达。免疫荧光显示狨猴 ES 细胞和植入前胚胎的细胞核中存在 SALL4。通过 RTPCR 和蛋白质印迹对 ES 细胞以及新生儿和成人睾丸中的狨猴 SALL4 亚型进行分析，结果显示两种不同的亚型：SALL4-A 和 SALL4-B。免疫组织化学将这种转录因子定位于产前和产后早期狨猴睾丸中原始生殖细胞和大多数生殖细胞的细胞核。在青春期和成年睾丸中，SALL4 存在于未分化的精原细胞中。在发育中的成人和小鼠睾丸中，SALL4 的表达模仿了狨猴的模式。其他 NHP 物种、树鼩、猫和狗的成年睾丸也在未分化的精原细胞中表现出 SALL4，表明在哺乳动物睾丸中存在保守表达。考虑到SALL4对小鼠发育的重要性，我们得出结论SALL4可能在哺乳动物生殖细胞发育过程中发挥重要作用，并参与成年睾丸精原细胞增殖的调节。版权所有 (C) 2012 S. Karger AG，巴塞尔
SALL4 (sal-like protein 4) is a pluripotency transcription factor, which is highly expressed in embryonic stem (ES) cells and which is essential for mouse preimplantation development. In adult mouse organs, Sall4 mRNA is highly expressed in the testis and ovary, while there is only little or no expression in other organs. There is also a high expression of SALL4 in human testicular germ cell tumors. However, there is as yet no detailed analysis of SALL4 expression during mammalian testicular development. We analyzed SALL4 expression in ES cells, preimplantation embryos, and the developing and adult testis of a nonhuman primate (NHP) species, the common marmoset monkey (Callithrix jacchus). Immunofluorescence revealed SALL4 in the nuclei of marmoset ES cells and preimplantation embryos. Marmoset SALL4 isoform analysis in ES cells and newborn and adult testis by RTPCR and Western blotting showed two different isoforms, SALL4-A and SALL4-B. Immunohistochemistry localized this transcription factor to the nuclei of primordial germ cells and most gonocytes in the prenatal and early postnatal marmoset testis. In the pubertal and adult testis SALL4 was present in undifferentiated spermatogonia. In the developing and adult human and mouse testis SALL4 expression mimicked the pattern in the marmoset. Adult testes from additional NHP species, the treeshrew, the cat and the dog also exhibited SALL4 in undifferentiated spermatogonia, indicating a conserved expression in the mammalian testis. Taking into account the importance of SALL4 for mouse development, we conclude that SALL4 may play an important role during mammalian germ cell development and is involved in the regulation of spermatogonial proliferation in the adult testis. Copyright (C) 2012 S. Karger AG, Basel