Nucleotide sequence and stability of the RNA component of RNase P from a temperature-sensitive mutant of E. coli.
Nucleotide sequence and stability of the RNA component of RNase P from a temperature-sensitive mutant of E. coli.
复制标题
大肠杆菌温度敏感突变体 RNase P 的 RNA 成分的核苷酸序列和稳定性。
作者：
H. Sakamoto;N. Kimura;F. Nagawa;Y. Shimura
从大肠杆菌RNase P活性缺陷的温度敏感突变株（ts 709）及其亲本野生型菌株（4273）中克隆了编码RNase P的RNA组分的基因，并测定了该基因及其侧翼区的完整核苷酸序列。确定RNA组分的5 '-和3'-末端序列，并绘制在DNA序列上。该突变基因在对应于RNA序列5 ′末端下游89和365个核苷酸的位置处具有GC-至-AT取代。与野生型RNA相比，突变体RNA的稳定性较差，在体内和体外降解迅速。
The gene coding for the RNA component of RNase P was cloned from a temperature-sensitive mutant of Escherichia coli defective in RNase P activity (ts709) and its parental wild-type strain (4273), and the complete nucleotide sequences of the gene and its flanking regions were determined. The 5'- and 3'-terminal sequences of the RNA component were determined and mapped on the DNA sequence. The mutant gene has GC-to-AT substitutions at positions corresponding to 89 and 365 nucleotides downstream from the 5' terminus of the RNA sequence. Comparing to the wild-type RNA, the mutant RNA is less stable and rapidly degraded in vivo and in vitro.