Tanshinone IIA induces growth inhibition and apoptosis in gastric cancer in vitro and in vivo
Tanshinone IIA induces growth inhibition and apoptosis in gastric cancer in vitro and in vivo
复制标题
丹参酮 IIA 在体外和体内诱导胃癌生长抑制和凋亡
DOI：
10.3892/or.2011.1524
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发表时间：
2012-02-01
期刊：
影响因子：
4.2
通讯作者：
Zhong, Liang
中科院分区：
文献类型：
--
作者：
Chen, Jian;Shi, Dong-Yun;Zhong, Liang
丹参酮IIA是一种从丹参根中提取的植物化学物质，具有抗炎和抗氧化活性。对乳腺癌、结肠癌、前列腺癌和肺癌的研究表明，丹参酮HA可能具有良好的抗肿瘤活性。然而，关于丹参酮IIA对胃癌细胞毒性作用的系统研究尚未见报道。本研究综合评价了丹参酮IIA对胃癌细胞和小鼠异种移植瘤模型的抗肿瘤作用。通过MTT法和流式细胞术分析体外细胞活力和凋亡情况。结果表明，丹参酮IIA可诱导胃癌细胞生长抑制和凋亡，并呈时间和浓度依赖性。进一步探讨丹参酮IIA诱导大鼠细胞凋亡的作用机制。我们发现丹参酮HA不仅能使细胞周期阻滞在G2/M期，还能触发细胞固有的凋亡信号通路。提示丹参酮IIA可作为治疗胃癌的有效辅助试剂。
As a phytochemical derived from the roots of Salvia miltiorrhiza Bunge, Tanshinone IIA has been reported to possess anti-inflammatory and antioxidant activity. Studies in breast, colon, prostate and lung cancer indicate that Tanshinone HA may exhibit a promising antitumor activity. However, systemic studies of the cytotoxic effects of Tanshinone IIA on gastric cancer have not been described. The present study offers a comprehensive evaluation of the antitumor effects of Tanshinone IIA in gastric cancer cells in vitro and in a mouse xenograft model. Cell viability and apoptosis in vitro were evaluated through the MTT assay and flow cytometry analysis. The results indicate that Tanshinone IIA can induce gastric cancer cell growth inhibition and apoptosis in a time- and concentration-dependent manner. Furthermore, we investigated the mechanism of the apoptotic effects induced by Tanshinone IIA. We found that Tanshinone HA can not only cause cell cycle arrest in the G2/M phase, but also trigger the intrinsic apoptotic signaling pathway. The results suggest that Tanshinone IIA may serve as an effective adjunctive reagent in the treatment of gastric cancer.