Clinical significance of the detection of thymidine phosphorylase activity in esophageal squamous cell carcinomas
Clinical significance of the detection of thymidine phosphorylase activity in esophageal squamous cell carcinomas
复制标题
DOI：
10.1159/000008713
复制
发表时间：
1999-07-01
影响因子：
1.6
通讯作者：
Kaibara, N
中科院分区：
文献类型：
--
作者：
Ikeguchi, M;Oka, S;Kaibara, N
在这项研究中，我们从1993年至1998年间接受食道切除术的患者(16例患者的转移淋巴结)获取新鲜的组织标本(肿瘤、邻近正常粘膜和转移的淋巴结)。用ELISA法测定肿瘤组织中的胸苷磷酸化酶(DThdPase)水平，并与肿瘤的临床病理结果进行比较，以评价其作为预后参数的价值。肿瘤组织dThdPase水平(2 2 1+/-2 1U/mg蛋白)和转移淋巴结组织(2 5 3+/-51U/mg蛋白)显著高于正常粘膜组织(53+/-7U/mg蛋白，p<0.001)。肿瘤组织中dThdPase水平与肿瘤组织学分级、肿瘤侵袭深度及有无淋巴结转移无关。32例dThdPase高表达患者的3年生存率(42%)与32例低dThdPase表达患者的3年生存率(52%)差异无统计学意义(P=0.267)。此外，肿瘤的dThdPase水平不是食道癌患者的独立预后因素。提示食道癌组织中dThdPase活性的升高可能与肿瘤的进展无关，不能作为判断食管鳞癌患者预后的有用指标。
In this study, we obtained fresh samples of tissue (tumors, adjacent normal mucosas, and metastatic lymph nodes) from 64 patients (metastatic lymph nodes were obtained from 16 patients) with esophageal cancer who underwent esophagectomy between 1993 and 1998. The thymidine phosphorylase (dThdPase) levels of tumors as determined by the ELISA method were compared with clinicopathological findings of tumo rs and were evaluated for their usefulness as a prognostic parameter for these patients. The dThdPase levels of tumors (221 +/- 21 U/mg protein) and metastatic lymph nodes (253 +/- 51 U/mg protein) were significantly higher than the dThdPase level of normal mucosas (53 +/- 7 U/mg protein, p < 0.001). The dThdPase levels of tumors did not correlate with the histopathological grading of tumors, the depth of tumor invasion, and lymph node metastasis. The 3-year survival rate of 32 patients with a high level of dThdPase (42%) was not different from that of 32 patients with a low level of dThdPase (52%, p = 0.267). Moreover, the dThdPase level of tumors was not an independent prognostic factor for patients with esophageal cancer. These findings suggest that the revel of dThdPase activity in esophageal cancers may not correlate with tumor progression and may not be a useful prognostic marker for patients with esophageal squamous cell carcinoma.