NUCLEOTIDE, NUCLEOSIDE AND BASE NUTRITIONAL-REQUIREMENTS OF THE MOSQUITO CULEX-PIPIENS
NUCLEOTIDE, NUCLEOSIDE AND BASE NUTRITIONAL-REQUIREMENTS OF THE MOSQUITO CULEX-PIPIENS
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DOI：
10.1016/0022-1910(79)90024-6
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发表时间：
1979-01-01
影响因子：
2.2
通讯作者：
DADD, RH
中科院分区：
文献类型：
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作者：
DADD, RH
在使用合成培养基的生长实验中研究了满足淡色隐球菌饮食核酸需求所需的核酸亚成分，其中核苷、碱基和替代核苷酸在3种核苷酸（腺苷酸、胸苷酸以及胞苷酸或尿苷酸）的混合物中被不同地取代，以前足以替代整个核酸。任何或全部3个核苷酸可以被相应的核苷取代而没有副作用，除了腺苷取代适度延迟化蛹之外。所有碱基取代均不令人满意：用胸腺嘧啶取代胸苷酸允许发育至成虫阶段，但速率大大降低；用腺嘌呤、胞嘧啶或尿嘧啶单一取代相应的核苷酸，与完全不存在核酸衍生物的情况相比，几乎无法实现更多的发育。肌苷酸或肌苷是腺苷酸的充分替代品，但乳清酸或乳清苷不能有效替代嘧啶核糖核苷酸、胞苷酸或尿苷酸。脱氧腺苷酸可以代替腺苷酸，尽管效率低下，但脱氧胞苷酸和脱氧尿苷酸对于相应的核糖核苷酸来说是非常差的替代品。所需的最少核酸衍生物似乎是嘌呤核糖核苷酸（腺苷酸或肌苷酸）、嘧啶核糖核苷（尿苷或胞苷）和嘧啶脱氧核糖核苷胸苷。
Nucleic acid subcomponents needed to satisfy the dietary nucleic acid requirement of C. pipiens were studied in growth experiments using synthetic media where nucleosides, bases and alternative nucleotides were variously substituted in mixtures of 3 nucleotides (adenylic acid, thymidylic acid and either cytidylic or uridylic acid), previously adequate replacements for whole nucleic acid. Any or all 3 nucleotides could be replaced by corresponding nucleosides without adverse effect, except that adenosine substitution moderately delayed pupation. All base substitutions were unsatisfactory: substitution of thymine for thymidylic acid allowed development to the adult stage but at a greatly reduced rate; single substitution of adenine, cytosine or uracil for the correponding nucleotides allowed scarcely more development than in the total absence of nucleic acid derivatives. Inosinic acid or inosine were adequate substitutes for adenylic acid, but orotic acid or orotidine were ineffective in place of the pyrimidine ribonucleotides, cytidylic or uridylic acids. Deoxyadenylic acid could take the place of adenylic acid, though inefficiently, but deoxycytidylic and deoxyuridylic acids were very poor replacements for the corresponding ribonucleotides. The minimal required nucleic acid derivatives appear to be a purine ribonucleotide (adenylic or inosinic acids), a pyrimidine ribonucleoside (either uridine or cytidine) and the pyrimidine deoxyribonucleoside, thymidine.