Is aquaporin-3 involved in water-permeability changes in the killifish during hypoxia and normoxic recovery, in freshwater or seawater?
Is aquaporin-3 involved in water-permeability changes in the killifish during hypoxia and normoxic recovery, in freshwater or seawater?
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在淡水或海水中，水通道蛋白 3 是否参与缺氧和常氧恢复期间鳉鱼的水渗透性变化？
DOI：
10.1002/jez.2393
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Grosell,Martin
中科院分区：
文献类型：
--
作者：
Ruhr,IlanM;Wood,ChrisM;Schauer,KevinL;Wang,Yadong;Mager,EdwardM;Stanton,Bruce;Grosell,Martin
水通道蛋白是脊椎动物中主要的水转运蛋白，但只有少数研究研究了水通道蛋白在鱼类中的功能，特别是在盐度挑战期间介导水渗透性。人们对缺氧（低氧）条件下水通道蛋白的功能知之甚少，而缺氧（低氧）条件下水通道蛋白的功能会深刻影响鳃功能。缺氧的鱼通常会扩大鳃表面积，缩小水到血液的扩散距离，以促进氧气进入血液。然而，这些改变鳃形态可能会导致不利的水和离子通量。因此，存在一种呼吸平衡，鱼类必须设法平衡高鳃气体交换与低离子和水渗透性。此外，海水和淡水硬骨鱼的鳃在渗透通量和离子通量方面具有显著不同的功能;因此，根据环境的盐度，缺氧可能具有非常不同的影响。本研究的目的是确定水通道蛋白在耐缺氧的广盐性普通鳉鱼（Fundulus heteroclitus）的水渗透性中发挥的作用，在两个重要的调节器官-鳃和肠中。使用免疫荧光，我们将水通道蛋白-3（AQP 3）蛋白分别定位于离子细胞和肠细胞的基底膜和顶膜。尽管缺氧增加了海水和淡水中鳃AQP 3信使RNA的表达，但蛋白质丰度并不相关。事实上，缺氧并没有改变水通道蛋白3蛋白在海水中的丰度，并减少它在淡水鳃细胞膜。总之，这些观察结果表明，鳉鱼AQP 3有助于减少淡水缺氧和常氧恢复期间的扩散水通量，并促进海水和淡水中的肠道渗透性。
Aquaporins are the predominant water‐transporting proteins in vertebrates, but only a handful of studies have investigated aquaporin function in fish, particularly in mediating water permeability during salinity challenges. Even less is known about aquaporin function in hypoxia (low oxygen), which can profoundly affect gill function. Fish deprived of oxygen typically enlarge gill surface area and shrink the water‐to‐blood diffusion distance, to facilitate oxygen uptake into the bloodstream. However, these alterations to gill morphology can result in unfavorable water and ion fluxes. Thus, there exists an osmorespiratory compromise, whereby fish must try to balance high branchial gas exchange with low ion and water permeability. Furthermore, the gills of seawater and freshwater teleosts have substantially different functions with respect to osmotic and ion fluxes; consequently, hypoxia can have very different effects according to the salinity of the environment. The purpose of this study was to determine what role aquaporins play in water permeability in the hypoxia‐tolerant euryhaline common killifish (Fundulus heteroclitus), in two important osmoregulatory organs—the gills and intestine. Using immunofluorescence, we localized aquaporin‐3 (AQP3) protein to the basolateral and apical membranes of ionocytes and enterocytes, respectively. Although hypoxia increased branchial AQP3 messenger‐RNA expression in seawater and freshwater, protein abundance did not correlate. Indeed, hypoxia did not alter AQP3 protein abundance in seawater and reduced it in the cell membranes of freshwater gills. Together, these observations suggest killifish AQP3 contributes to reduced diffusive water flux during hypoxia and normoxic recovery in freshwater and facilitates intestinal permeability in seawater and freshwater.
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